Glucose, total NEFA, and TG were measured as previously described (29 (link)). Plasma C-peptide, insulin, and leptin were measured using Luminex xMAP-based immunoassays (Millipore, Etobicoke, Ontario, Canada). HOMA of IR (HOMA-IR) was also calculated as an alternative index of IR as fasting plasma insulin (units/L) × fasting plasma glucose (mmol/L)/22.5. Insulin secretion rate (ISR) was determined via deconvolution of plasma C-peptide levels with standard two-compartmental kinetic parameters (30 (link)). The disposition index (DI), an index of β-cell function, was determined by the product of the postprandial AUC, ISR/AUC glucose, and Matsuda index (25 (link),31 (link)).
Luminex xmap based immunoassays
Manufactured by Merck Group
Sourced in Canada
Luminex xMAP-based immunoassays are a multiplex platform that uses color-coded magnetic microspheres to analyze multiple analytes simultaneously in a single sample. The technology allows for the detection and quantification of proteins, peptides, and other biomolecules.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using luminex xmap based immunoassays
1
Metabolic Biomarkers and β-cell Function
2
Insulin Resistance Indices and Metabolic Markers
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Glucose, total NEFA, and TG were measured as described.126 (link) Plasma adiponectin, insulin, and leptin were measured using Luminex xMAP-based immunoassays (Millipore, Etobicoke, ON, Canada). The Matsuda index was calculated as previously described.127 (link) Homeostasis model assessment of IR (HOMA-IR) was also calculated as an alternative index of insulin resistance as fasting plasma insulin (U/L) × fasting plasma glucose (mmol/L)/22.5. Fasting and postprandial AT insulin resistance (ADIPO-IR) were computed as plasma insulin (pmol/L) × plasma NEFA (mmol/L) at the fasting state and at 120 min, respectively.16 (link),17 (link) Plasma appearance rates of palmitate (Rapalmitate) and total NEFA (RaNEFA) were calculated with the Steele’s non-steady-state equations128 (link) as previously described.4 (link),95 (link) Adipose Tissue Insulin Resistance Index (ATIRI) was calculated as the reduction in the RaNEFA divided by change in plasma insulin from 0 to 120 min.15 (link)
3
Comprehensive Metabolic Profiling Protocol
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Glucose, insulin, total NEFA TG, plasma cortisol, TSH, free T3 and free T4 were measured using specific radioimmunoassays and colorimetric assays14 (link)36 (link). Plasma C-peptide, GIP, total GLP-1, glucagon, insulin and leptin and PYY were measured using Luminex xMAP-based immunoassays (Millipore, Etobicoke, ON, Canada). ACTH, GH, adiponectin and acylated ghrelin were measured by ELISA (Alpco, Salem, NH, USA). Individual plasma NEFA (palmitate, linoleate, oleate), [U-13C]palmitate enrichment, [7,7,8,8-2H4]palmitate enrichment and [1,1,2,3,3-2H]glycerol enrichment were measured by gas chromatography–mass spectrometry36 (link). [3-3H]-glucose specific activity was determined by liquid scintillation spectrometry35 (link).
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