mRNA was isolated from cells using RNeasy Plus Universal Kits (Qiagen) and reverse transcribed using Qiagen Onestep RT-PCR kits (Qiagen). mRNA levels were determined by quantitative PCR (qPCR) using TaqMan probes and reagents. The cyclophilin gene was used as a normalization control and experimental data were compared with EV-treated samples as described. A pathway focused RT2 Profiler PCR Array for Human Cell Motility array (Qiagen) was performed according to the manufacture’s instructions (GEO accession no. GSE101159). miRNAs were prepared using miRCURY Cells and Tissue RNA, Biofluids and Cell and Plant isolation kits, and Universal cDNA was synthesis kits II (Exiqon), and transcript levels were quantified using miRCURY LNA microRNA PCR, ExiLENT SYBR master mix with ready-to-use PCR, human panel I and II or cel-miR-39-3p, LNA control primer set kits (Exiqon) according to the manufacture’s protocol.
Mircury lna microrna pcr
Manufactured by Qiagen
The MiRCURY LNA microRNA PCR is a laboratory equipment product designed for the quantitative detection and analysis of microRNA expression levels. It utilizes Locked Nucleic Acid (LNA) technology to provide sensitive and specific quantification of microRNA targets.
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Lab products found in correlation
2 protocols using mircury lna microrna pcr
1
Investigating Cell Motility Pathways via qPCR and miRNA Analysis
2
Saliva miRNA Profiling Protocol
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Pools of saliva (described in the result section) were screened for dysregulated miRNA primarily using serum plasma focused miRCURY LNA™ microRNA PCR (Exiqon, Inc.). The panel is chosen as miRnome of saliva was previously found to be significantly overlapping with those from serum/plasma [12 (link)]. cDNA synthesized were diluted 40 folds in water and 2 μl is added to each well of qPCR plates. Quantitative real-time PCR reactions were carried out according to manufacture protocol using ExiLENT SYBR® Green master mix (Exiqon, Inc.).
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