Proteins (insulin, glucagon, and somatostatin) were extracted from 50 IEQs using 300 µL RIPA buffer (Sigma-Aldrich) containing protease and phosphatase inhibitor (Nacalai Tesque, Inc., Kyoto, Kyoto, Japan). Insulin content was measured using the enzyme-linked immunosorbent assay (ELISA) with the Mouse Insulin ELISA Kit (RTU) (FUJIFILM Wako Shibayagi Co., Shibukawa, Gumma, Japan). Somatostatin content was determined using the Somatostatin ELISA kit (Phoenix Pharmaceuticals, Inc., Burlingame, CA, USA). iMark Plate Reader (Bio-Rad Laboratories, Inc.) at OD450 with Microplate Manager Software (ver. 6.3, Bio-Rad Laboratories, Inc.) was used for reading sample absorbance.
Somatostatin elisa kit
Manufactured by Phoenix Pharmaceuticals
Sourced in United States
The Somatostatin ELISA kit is a laboratory equipment product designed to detect and quantify somatostatin, a hormone involved in the regulation of various physiological processes. This kit utilizes the enzyme-linked immunosorbent assay (ELISA) technique to measure somatostatin levels in biological samples.
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Lab products found in correlation
3 protocols using somatostatin elisa kit
1
Quantification of Pancreatic Hormones
2
Serum Hormone Quantification via ELISA
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Insulin, glucagon, GLP-1, and leptin ELISA kits were obtained from Crystal Chem (#90080, #81518, #81508, #90030). The somatostatin ELISA kit was obtained from Phoenix Pharmaceuticals (EK-060-03). Mouse whole blood was collected via tail tip, and serum was isolated after clotting by centrifuging at 10,000 rpm for 10 min. Between 5 and 20 μL of serum or secretion sample were used for ELISA per the manufacturer’s protocol. Each sample was assayed in triplicate.
3
Blood Serum Analysis in Fasted Mice
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The mice were fasting 4 hours before collection of the whole blood. The serum was collected by centrifuge at 2,000 x g for 10 minutes at 4°C. The ELISA was preformed according to the manual. The leptin ELISA kit (catalog # 90030) was purchased in Crystal Chem, IGF‐1 ELISA kit (catalog # MG100) was R&D SYSTEM, the high‐molecular‐weight adiponectin ELISA kit (catalog # 47‐ADPMS‐E01) was ALPCO, and somatostatin ELISA kit (EK‐060–03) was phoenix pharmaceuticals.
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