Sirius red was used to stain for collagen. Immunohistochemistry was performed on paraffin-embedded liver sections. Antibodies against HNF1α (ab96777, Abcam), α-SMA (BM0002, Boster, Wuhan, China), SHP-1 (ab2020, Abcam), p-Erk1/2 (Thr202/Tyr204, 4370, Cell Signaling), p-p65 (sc-101752, Santa Cruz) and p-STAT3 (Ser727, 9134, Cell Signaling) were used for immunohistochemistry. Sections were stained with ImmunoCruz™ goat ABC Staining (Sc-2023, Santa Cruz) or EnVision Detection Rabbit/Mouse Kit (GK500710, GeneTech, Shanghai, China) and counterstained with hematoxylin. Areas of positive stained sites were measured using image analyses software Image-Pro Plus 6.0 (Media Cybernetics). Percentage of positive area in corresponding field of liver tissue was calculated to show the intensity of collagen deposition or protein expression.
Sc 101752
Manufactured by Santa Cruz Biotechnology
Sourced in China
Sc-101752 is a laboratory instrument used for the separation and analysis of biological samples. It functions as a high-performance liquid chromatography (HPLC) system, enabling the separation of complex mixtures into their individual components. The core function of Sc-101752 is to provide accurate, reliable, and efficient separation and quantification of various biomolecules, such as proteins, peptides, and small molecules, in research and analytical settings.
Automatically generated - may contain errors
Lab products found in correlation
Image pro plus 6, by Media Cybernetics (1 mentions)
Envision detection rabbit mouse kit, by Gene Tech (1 mentions)
Ab96777, by Abcam (1 mentions)
Ab2020, by Abcam (1 mentions)
Bm0002, by Boster Bio (1 mentions)
Ripa lysis buffer, by Beyotime (1 mentions)
Bca protein quantitative kit, by Beyotime (1 mentions)
Enhanced chemiluminescence kit, by Beyotime (1 mentions)
Ab32518, by Abcam (1 mentions)
Ab175329, by Abcam (1 mentions)
Sc 52943, by Santa Cruz Biotechnology (1 mentions)
Ab16502, by Abcam (1 mentions)
Ab8245, by Abcam (1 mentions)
2 protocols using sc 101752
1
Quantitative Liver Fibrosis Assessment
2
Western Blotting Quantification of Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total protein was released from tissues and cells by RIPA lysis buffer (Beyotime, Shanghai, China). The concentration of extracted protein was determined by BCA Protein quantitative Kit (Beyotime, Shanghai, China). The electrophoresis and transferring to PVDF membrane were performed in a Bis–Tris Gel system (Bio-Rad, Hercules, CA, USA). The blots were cut prior to hybridization with antibody, and incubated overnight at 4 ℃ with primary antibodies against RAP1 (#ab175329, Abcam, Cambridge, UK), IκBα (#ab32518, Abcam), p-IκBα (#sc-52943, Santacruze, Shanghai, China), p-NFκB p65 (#ab16502, Abcam), p-NFκB p65(Ser 536) (#sc-101752, Santacruze) and GAPDH (#ab8245, Abcam). After washing for 3 times, the blots were incubated with goat anti-mouse IgG secondary antibodies (#ab97040) for 1 h at 37 °C. The proteins were visualized by using enhanced chemiluminescence kit (Beyotime), and then quantified by ImageJ Software.
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