The human malignant melanoma cell line WM-266-4 and the mouse macrophage cell line RAW264.7 were procured from the Korean Cell Line Bank. The cells were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 100 U/ml penicillin, 100 µg/ml streptomycin, and 10% heat-inactivated fetal bovine serum. The culture reagents used in this study were purchased from Welgene. The cell lines were subcultured in 100-mm cell culture plates (Sarstedt), with fresh DMEM replaced every three days. All cells were cultured at 37°C in a humidified atmosphere containing 5% CO2.
Wm 266 4
Manufactured by Korean Cell Line Bank
The WM-266-4 is a cell line derived from a human melanoma. It is used for research purposes in the field of oncology and cell biology. The core function of the WM-266-4 cell line is to serve as a model system for the study of melanoma and related cancer research.
Automatically generated - may contain errors
Lab products found in correlation
100 mm cell culture plates, by Sarstedt (1 mentions)
Raw 264, by Korean Cell Line Bank (1 mentions)
Sk mel 28, by Korean Cell Line Bank (1 mentions)
Minimum essential medium (mem), by Welgene (1 mentions)
Pathscan stress and apoptosis signaling antibody array kit, by Cell Signaling Technology (1 mentions)
Sk mel 2, by Korean Cell Line Bank (1 mentions)
Sk mel 2, by Welgene (1 mentions)
G 361, by Welgene (1 mentions)
Sk mel 5, by Korean Cell Line Bank (1 mentions)
Human phospho receptor tyrosine kinase array kit, by R&D Systems (1 mentions)
Fetal bovine serum (fbs), by Welgene (1 mentions)
2 protocols using wm 266 4
1
Culturing Human Melanoma and Mouse Macrophage Cells
2
Melanoma Cell Line Characterization
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Human melanoma cell lines (G361, WM-266-4, SK-MEL-2, SK-MEL-5, SK-MEL-28) were purchased from Korean Cell Line Bank (Seoul, South Korea). G361, SK-MEL-2 and SK-MEL-5 were cultured in RPMI and WM-266-4 and SK-MEL-28 were cultured in minimum essential medium (MEM) (Welgene Inc., South Korea). The cell lines were maintained at 37°C and 5% CO2 in media supplemented with 10% fetal bovine serum (Welgene Inc, South Korea) and 1% of antibiotics (Gibco/Invitrogen, Carlsbad, CA). The PathScan Stress and Apoptosis Signaling Antibody Array Kit (#12856) was purchased from Cell Signaling Technology (Danvers, MA, USA). Human Phospho-Receptor Tyrosine Kinase (RTK) Array Kit (#ARY001B) were purchased from R&D Systems (Minneapolis, MN, USA).
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