Uplc g2 xs qtof spectrometer

All commercially available materials and reagents were used without purification unless otherwise indicated. Purification via column chromatography was performed using silica gel (200–300 mesh). The melting points of the target compounds 10a–10l and 14a–14n were determined using an x-5 micro melting point apparatus, which was uncorrected. The purity and characterization of the target compounds were established using a combination of high-performance liquid chromatography and NMR analytical techniques, and the purity was >95% for all test compounds. NMR spectra (500 MHz for ¹H NMR and 125 MHz for ¹³C NMR spectra) were recorded on a Bruker AVANCE NEO 500 instrument, and were to be determined in CDCl₃ or DMSO-d₆. Chemical shifts were reported in ppm relative to tetramethylsilane (0.00 ppm) or solvent peaks as the internal reference. Splitting patterns are indicated as follows: s, singlet; d, doublet; t, triplet; m, multiplet. Coupling constants (J values) are given in hertz (Hz). High resolution mass spectrometry was conducted using a UPLC G2-XS QTOF spectrometer (Waters) with the electrospray ionization Fourier transform ion cyclotron resonance technique. The NMR and HRMS spectra of compounds 10a–10l and 14a-14n are presented in Figures S3–S80.

All of the commercially available materials and reagents were used without purification unless otherwise indicated. Purification by column chromatography was performed using silica gel (200–300 mesh). The melting points of the target compounds 1f–7f and 11a–11h were determined using an x-5 micro melting point apparatus, which was uncorrected. The NMR spectra (500 MHz for ¹H-NMR and 125 MHz for ¹³C-NMR spectra) were recorded using a Bruker AVANCE NEO 500 instrument (Bruker, Germany, compounds were dissolved in DMSO_d6). Chemical shifts are shown as values relative to the internal standard (tetramethylsilane), and coupling constants (J values) are given in hertz (Hz). High-resolution mass spectrometry was conducted using an UPLC G2-XS Qtof spectrometer (Waters, Milford, MA, USA). The names of the compounds were obtained by ChemBioDraw Ultra 14.0 (Cambridge Soft, Cambridge, MA, USA). The NMR and HRMS spectra of compounds 1f–7f and 11a–11h are presented in Supplementary Materials Figures S1–S45.