Tregs, CD8+ T cells as well as 4T1 cells were obtained as described above and co-cultured in RPMI-1640 complete culture medium at a ratio of 1:10:2. After treatment as indicated, the non-adherent T cells were harvested. The proliferation and apoptosis of the CD8+ T cell population were detected by CFSE-PI staining and flow cytometry. The expression levels of perforin and granzyme B in CD8+ T cells were analyzed by flow cytometry using the PE-conjugated perforin antibody (12-9392-82, eBioscience) and APC-conjugated granzyme B antibody (17-8898-82, eBioscience). The adherent 4T1 cells were harvested and subjected to LDH activity detection using the LDH Activity Kit (A020-2-2, Jiancheng Bioengineering Institute).
Ldh activity kit
Manufactured by Nanjing Jiancheng
Sourced in China
The LDH activity kit is a laboratory instrument used to measure the activity of the enzyme lactate dehydrogenase (LDH) in biological samples. It provides a quantitative assessment of LDH levels, which can be an important indicator of various medical conditions.
Automatically generated - may contain errors
3 protocols using ldh activity kit
1
Cytotoxic CD8+ T Cell Assay
2
Plasma Enzymes and Glucose Measurement
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Plasma CK, LDH activity, and glucose were measured with CK kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China), LDH activity kit (Nanjing Jiancheng Bioengineering Institute) and glucose oxidase kit (Nanjing Jiancheng Bioengineering Institute), respectively. Each blood sample was measured in triplicate according to the manufacturers’ instructions.
3
Cell Viability Assay for Hyperoxia-Induced A549 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To measure the cell viability, hyperoxia-induced A549 cells were seeded in 96-well plates. Then, 10 µL of cell counting kit-8 (CCK-8) reagents (BD Biosciences, Franklin Lakes, NJ, USA) were separately added into each well of the 96-well plate. The optical density 450 nm (OD450) was measured using a microplate reader (BioTek Instruments Inc., Winooski Vermont, WV, USA). Lactate dehydrogenase (LDH) released in the cell culture media was monitored by using the LDH activity kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China). LDH release is presented as % cytotoxicity.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!