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Waters acquity 1 class uplc

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The Waters Acquity I-Class UPLC is a high-performance liquid chromatography (HPLC) system designed for efficient and reliable separation and analysis of a wide range of chemical compounds. It utilizes ultra-high pressure technology to deliver superior chromatographic resolution and sensitivity.

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Lab products found in correlation

Xevo tq xs triple quadrupole mass spectrometer, by Waters Corporation (3 mentions) Acquity uplc beh c18, by Waters Corporation (1 mentions) Acquity uplc hss t3, by Waters Corporation (1 mentions) Xevo g2 xs qtof, by Waters Corporation (1 mentions) Xevo tq s micro triple quadrupole mass spectrometer, by Waters Corporation (1 mentions) Oasis max uelution plate, by Waters Corporation (1 mentions) Q exactive orbitrap ms, by Thermo Fisher Scientific (1 mentions) Sep pak c18, by Waters Corporation (1 mentions) Acquity beh c18 column, by Waters Corporation (1 mentions)

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Acquity UPLC (716 citations) ACQUITY UPLC I-Class (163 citations) Waters Acquity UPLC (80 citations) Acquity I-Class (28 citations) Waters Acquity UPLC I-Class system (15 citations) UPLC I-Class (13 citations) Waters I-Class UPLC (3 citations)

6 protocols using waters acquity 1 class uplc

1

UPLC-MS/MS Analysis of Analytes

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LC/MS was performed on a Waters Xevo TQ-XS triple quadrupole mass spectrometer connected to a Waters Acquity I-Class UPLC (Waters Corp., Milford, MA USA). Separation of analytes was obtained using a Waters Acquity UPLC BEH C18 (1.0 × 100 mm, 1.7um particle size) with using water containing 0.01 % acetic acid (mobile phase A) and acetonitrile (mobile phase B). The LC gradient and MS settings are described in the Supplemental Materials.
Milne G.L., Nogueira M.S., Gao B., Sanchez S.C., Amin W., Thomas S., Oger C., Galano J.M., Murff H.J., Yang G, & Durand T. (2024). Identification of novel F2-isoprostane metabolites by specific UDP-glucuronosyltransferases. Redox Biology, 70, 103020.
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2

UPLC-MS/MS Quantification of Analytes

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LC/MS was performed on a Waters Xevo TQ-XS triple quadrupole mass spectrometer connected to a Waters Acquity I-Class UPLC (Waters Corp., Milford, MA USA). Separation of analytes was obtained using a Waters Acquity UPLC HSS T3 (1.0 × 100 mm, 1.8 μm particle size) with mobile phases A and B as described above. The LC gradient and MS settings are described in the Supplemental Materials.
Milne G.L., Nogueira M.S., Gao B., Sanchez S.C., Amin W., Thomas S., Oger C., Galano J.M., Murff H.J., Yang G, & Durand T. (2024). Identification of novel F2-isoprostane metabolites by specific UDP-glucuronosyltransferases. Redox Biology, 70, 103020.
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3

Accurate Mass Measurement of Samples by UPLC-QTOF-MS

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Accurate mass data
of samples were obtained using HRMS system with Waters Acquity I Class
UPLC and Xevo G2-XS Q-TOF (Waters Corp., Milford, MA, USA). The samples
were separated on reversed phase ACQUITY UPLC BEH C18 column (2.1
× 50 mm, 1.7 μm) using gradient elution with 0.1% FA in
H2O as mobile phase A and 0.1% FA in MeCN as mobile phase
B. Acquity PDA Detector was in the range 210–400 nm. The injection
volume was 0.1 μL. Analytes were separated by a gradient method
(Supporting Information, Table S5). The
column temperature was set at 40 °C and the flow rate at 0.4
mL/min. Instrument control and accurate mass data were processed using
Masslynx software. The MS data were acquired in positive ionization
mode with the following conditions: MS equipped with ESI, sensitivity
mode, capillary voltage at 2.5 kV, sampling cone voltage at 40.0 V,
source temperature at 100 °C, cone gas flow at 50 L/h, desolvation
gas flow at 600.0 (L/h), acquisition mass range of 50–1200
Da.
Michaelides I.N., Collie G.W., Börjesson U., Vasalou C., Alkhatib O., Barlind L., Cheung T., Dale I.L., Embrey K.J., Hennessy E.J., Khurana P., Koh C.M., Lamb M.L., Liu J., Moss T.A., O’Neill D.J., Phillips C., Shaw J., Snijder A., Storer R.I., Stubbs C.J., Han F., Li C., Qiao J., Sun D.Q., Wang J., Wang P, & Yang W. (2023). Discovery and Optimization of the First ATP Competitive Type-III c-MET Inhibitor. Journal of Medicinal Chemistry, 66(13), 8782-8807.
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4

Quantitative Eicosanoid Profiling from Media

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To quantify eicosanoids in media samples, 100uL of media was placed in a microcentrifuge tube containing 25% methanol in water (500uL) and internal standard (d4-PGE2 and d4-LTB4, 1ng each). The sample was vortexed and then extracted on an Oasis MAX uElution plate (Waters Corp., Milford, MA) as follows. Sample wells were first washed methanol (200uL) followed by 25% methanol in water (200uL). The sample was then loaded into the well and washed with 600uL 25% methanol. Eicosanoids were eluted from the plate with 30uL 2-propanol/acetonitrile (50/50, v/v) containing 5% formic acid into a 96-well elution plate containing 30uL water in each well.
Samples were analyzed on a Waters Xevo TQ-S micro triple quadrupole mass spectrometer connected to a Waters Acquity I-Class UPLC (Waters Corp., Milford, MA USA). Separation of analytes was obtained using an Acquity PFP column (2.1 x 100mm) with mobile phase A being 0.01% formic acid in water and mobile phase B acetonitrile. Eicosanoids were separated using a gradient elution beginning with 30% B going to 95% B over 8 minutes at a flow rate of 0.250mL/min.
Montenegro-Burke J.R., Sutton J.A., Rogers L.M., Milne G.L., McLean J.A, & Aronoff D.M. (2016). Lipid Profiling of Polarized Human Monocyte-derived Macrophages. Prostaglandins & other lipid mediators, 127, 1-8.
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5

Metabolite Extraction and Analysis Protocol

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Metabolite extractions were performed as in Boysen et al. (2018 (link)), using a modified Bligh–Dyer extraction that yielded an aqueous and organic fraction. Aqueous soluble metabolites were analysed with two injections for each sample, one with a spike of heavy isotope labelled internal standards and one with an equal volume spike of UPLC grade water. Samples were run using both HILIC and reversed‐phase (RP) chromatography on a Waters Acquity I‐Class UPLC (Waters Corporation) with injection volumes of 2 and 15 μl respectively, coupled to a Q Exactive Orbitrap MS (Thermo Scientific). Details of the chromatography and mass spectrometry methods are in Boysen et al. (2018 (link)) and in the Supplemental Methods.
Boysen A.K., Durham B.P., Kumler W., Key R.S., Heal K.R., Carlson L.T., Groussman R.D., Armbrust E.V, & Ingalls A.E. (2022). Glycine betaine uptake and metabolism in marine microbial communities. Environmental Microbiology, 24(5), 2380-2403.
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6

Targeted Eicosanoid Profiling in Urine

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After thawing, internal standards (3ng) were added to urine samples (0.5mL), and the sample was treated with methyloxime HCl to stabilize PGE-M, TN-E, PGD-M, and PGI-M. Following derivatization, the analytes were extracted using a C-18 Sep-Pak (Waters Corp. Milford, MA USA) and eluted with ethyl acetate. The samples were dried under a stream of dry nitrogen and then reconstituted in 75μL mobile phase A for ultra-pressure liquid chromatography/electrospray ionization tandem mass spectrometry (UPLC/ESI-MS/MS) analysis. LC was performed on a 2.0 x 50 mm, 1.7μm particle Acquity BEH C18 column (Waters Corporation, Milford, MA, USA) using a Waters Acquity I-Class UPLC. Mobile phase A was 95/5/0.01 (v/v/v) water/acetonitrile/acetic acid, and mobile phase B was 10/90/0.01 (v/v/v) water/acetonitrile/acetic acid. Samples were separated by a gradient of 85–5% of mobile phase A over 12 min at a flow rate of 0.375 mL/min prior to delivery to a Waters Xevo TQ-XS triple quadrupole mass spectrometer. Data were analyzed using MassLynx software and values were calculated in ng/mL.
Arriaga M.B., Karim F., Queiroz A.T., Araújo-Pereira M., Barreto-Duarte B., Sales C., Moosa M.Y., Mazibuko M., Milne G.L., Maruri F., Serezani C.H., Koethe J.R., Figueiredo M.C., Kritski A.L., Cordeiro-Santos M., Rolla V.C., Sterling T.R., Leslie A, & Andrade B.B. (2022). Effect of Dysglycemia on Urinary Lipid Mediator Profiles in Persons With Pulmonary Tuberculosis. Frontiers in Immunology, 13, 919802.
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