Soybean protein isolate

Soybean protein isolate, pectin, quercetin and methanol were purchased from Shanghai Yuanye Biotechnology Co., Ltd., Shanghai, China. Extra olive oil was donated by Chongqing Jiangyuan Olive Development Co., Ltd., Chongqing, China. Hydrochloric acid and sodium hydroxide were obtained from National Medicine Group Chemical Reagent Co., Ltd., Shanghai, China. Artificial gastric fluid, artificial intestinal fluid, artificial saliva, Nile red dye and Nile blue dye were provided by Beijing Leigen Biotechnology Co., Ltd., Beijing, China.

Food-grade TPSO was supplied by Heze Ruipu Peony Industry Technology Development Co., Ltd. (Heze, Shandong, China). The composition of major fatty acids and beneficial components in TPSO was shown in Table S1. Whey protein isolate (WPI, food grade) was purchased from Zhengzhou Weifeng Biotechnology Co., Ltd. (Zhengzhou, Henan, China). Soybean protein isolate (SPI, biological reagent grade), mucin (porcine stomach, biological reagent grade), pepsin (porcine gastric mucosa, 30,000 U/g), bile salt (pig, cholic acid content ≥ 60%), and pancreatin (pancreatic lipase activity ≥ 4000 U/g) were supplied by Yuanye Biological Technology Co., Ltd. (Shanghai, China). Nile red was purchased from Shanghai Macklinn Biochemical Technology Co., Ltd. (Shanghai, China). Gelatin (GE, chemical pure grade), sodium alginate (SA, chemical pure grade), gum arabic (GA, biological reagent grade), sodium carboxymethyl cellulose (CMC-Na, chemical pure grade), sodium benzoate, and other reagents of analytical grade were obtained from Sinopharm Chemical Reagents Co., Ltd. (Shanghai, China).