2 dodecanone

Manufactured by Merck Group

2-dodecanone is a chemical compound that can be used as a laboratory reagent. It is a colorless liquid with a characteristic odor. 2-dodecanone is commonly used in various chemical and analytical applications, but a detailed description of its core function would require more specific information that is not available to me.

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3 protocols using 2 dodecanone

Ketone-Functionalized Liposome Synthesis

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To form liposome solutions
bearing ketone functionalities, into
a 5 mL vial was added 120 μL of 2-dodecanone (Sigma-Aldrich)
(10 mg/mL in CHCl₃), followed by 454 μL of POPC (Avanti
Polar Lipids) (10 mg/mL in CHCl₃) followed by 10 μL
of DOTAP (Avanti Polar Lipids) (10 mg/mL in CHCl₃), which
were then allowed to evaporate over 24 h. Once the CHCl₃ is evaporated, 3 mL of fresh PBS (Sigma-Aldrich) is added, followed
by tip sonication. Tip sonication of the lipid suspensions was carried
out over 10 min in a 23 °C water bath and stored at 4 °C
at 30 W. To apply ketone cell surface engineering to cultured cells,
they need to be cultured with a confluency between 75 and 80%; the
cell culture medium was then aspirated, followed by the addition of
fresh serum containing medium (2 mL), and 5% v/v ketone liposome suspension
(100 μL) was added and incubated with the cells under growth
conditions for 5 min followed by three 3 mL washes of PBS and addition
of fresh growth medium to the cells awaiting transfection.

O’Brien P.J., Elahipanah S., Rogozhnikov D, & Yousaf M.N. (2017). Bio-Orthogonal Mediated Nucleic Acid Transfection of Cells via Cell Surface Engineering. ACS Central Science, 3(5), 489-500.

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Acetoin and Plant Root Development

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1-N-naphthylphthalamic acid (NPA) and yucasin were dissolved in DMSO to make a 50 mM stock solution. For treatment, the required amount of the stock solutions was added into MS salts agar and mixed in uniform before being poured into Petri dishes. Acetoin ([?] 98%, Sigma-Aldrich) was dissolved in DDW to make a 250 mM stock solution and was supplied at different doses (0, 10, 30, 100, 300 and 1000 μM) to the plant growth medium. Petri dishes containing 5 plants under different treatments were placed in growth chamber for 8 d to estimate root development. For investigating the effects of Acetoin on CYCB1 expression pattern, prebranch sites formation and LRPs development stage, 3-day-old seedlings were grown with 30 μM Acetoin for 6 d.
To test the effects of other VCs from SQR9 on root development, the pure compounds (dodecane, tetradecane, 2-dodecanone, 2-nonanone, pentadecane, 2-undecanone, 2-heptanone) were purchased from Sigma. Arabidopsisseedlings were exposed to a filter paper added with 0, 1, 10 and 100 μl pure compound in a bi-compartmented Petri dish for 8 d to estimate root development.

Li Y., Shao J., Xie Y., Jia L., Y F., Xu Z., Zhang N., Feng H., Xun W., Liu Y., Shen Q., Xuan W., & Zhang R. (2020). Volatile compounds from beneficial rhizobacteria Bacillus spp. promote periodic lateral root development in Arabidopsis.

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Characterization of Polyisobutylene Succinic Anhydride

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Chemicals. Acetone (HPLC grade, Caledon), hexane (HPLC grade, Caledon), xylene (reagent grade, 98.5%, EMD), deuterated chloroform (CDCl3, 99.8%, Cambridge Isotope Laboratories, Inc.), tetrahydrofuran (THF, HPLC grade, Caledon), dodecane (anhydrous, 99%, Sigma-Aldrich), ethyl ether (anhydrous, 99% Sigma-Aldrich), ethyl acetate (HPLC, 99.7% Sigma-Aldrich), and 2-dodecanone (GC grade, 97%, Sigma-Aldrich) were used as received. The chemicals hexamethylenediamine (HMDA, 98%), diethylenetriamine (DETA, 99%), triethylenetetramine (TETA, 97%), tetraethylenepentamine (TEPA, technical grade), pentaethylenehexamine (PEHA, technical grade), di butylamine (DBA, 99.5%), octylamine (99%), N-methylsuccinimide (N-MSI, 99%), butylamine (BUA, 99%), diethylamine (DEA, 99.5%), triethylamine (TEA, 99.5%), ethylene carbonate (EC, 98%), magnesium sulfate anhydrous (97%), and activated charcoal (100 mesh) were purchased from Sigma-Aldrich and were employed without further purification. Polyisobutylene succinic anhydride (PIBSA) was supplied by Imperial Oil. The chemical composition of this sample was characterized by NMR, GPC, and FTIR analysis and found to contain on average one SA unit per 52 ± 2 isobutylene monomers. Assuming one SA moiety per chain, this PIBSA sample would have an Mn of 3,012 g.mol  equivalent to a total acid number (TAN) of 37.2 mg of KOH per gram of PIBSA.

Pirouz S., Wang Y., Chong J.M, & Duhamel J. (2015). Chemical Modification of Polyisobutylene Succinimide Dispersants and Characterization of Their Associative Properties. The journal of physical chemistry. B, 119(37).

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