Diethylenetriamine deta

Bisphenol A (BPA) used as A₂ monomer for preparing the in-situ generated DGEBA monomer was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan) and was purified by recrystallization from toluene before using. Epichlorohydrin (ECH) as epoxidation reagent was obtained from Tokyo Chemical Industry Co., Ltd. Pentaerythritol (PE) used as B₄ branch generating unit for reacting with the in-situ generated DGEBA was purchased from Tokyo Chemical Industry Co., Ltd., Japan and was purified by recrystallization from ethanol prior to use. Polyethylene glycol (PEG400, Mw = 400 g/mol) as A₂ monomer was purchased from Sigma-Aldrich, St. Louis, MI, USA. Sodium hydroxide (NaOH) as base catalyst and sodium chloride (NaCl) were obtained from Ajax Finechem, Australia. Hydrobromic acid (HBr), acetic acid, potassium hydrogen phthalate, methyl violet, and chlorobenzene were used to determine epoxy equivalent weight (EEW) of the hyperbranched epoxy resins and were purchased from Tokyo Chemical Industry Co., Ltd., Japan. Diethylenetriamine (DETA) as amine curing agent was brought from Tokyo Chemical Industry Co., Ltd., Japan. Solvents were analytical grades and used as received.

Methoxy poly (ethylene glycol)-amine (mPEG-NH₂₎ (M_n = 5000 g/mol) and NH₂-PEG-NH₂ (M_n = 5000 g/mol) were purchased from Lasan Bio Inc., (Arab, AL, USA). L-glutamic acid γ-benzyl ester (BLG), 2-hydroxypyridine (2-HP), pyridine, N,N′-dicyclohexylcarbodiimide (DCC), folic acid (FA), chloroform, dimethyl sulfoxide (DMSO), N,N′-dimethylformamide (DMF), and tetrahydrofuran (THF) were purchased from Sigma Aldrich (St. Louis, MO, USA). Triphosgene, diethylenetriamine (DETA), and pentaethylenehexamine (PEHA) were obtained from Tokyo Chemical Industry (Tokyo, Japan).