O phtaldialdehyde

All chemicals were of analytical grade unless otherwise stated. More specifically, tetra-methyl-ammonium-hydroxide (TMAH) 25%, sodium hydroxide, o-phtaldialdehyde and butylated hydroxyToluene were purchased from Sigma-Aldrich (Steinheim, Germany). Toluene, hydrogen chloride, methanol, chloroform, sodium chloride, and 20% boron trifluoride were purchased from Merck (Darmstadt, Germany). n-Heptane was purchased from VWR (Radnor, Pennsylvania, USA). The purified C23:0 was purchased from Nu Chek Prep (Elysian, MN, USA).

The substances utilized for cell culture and reagent-grade chemicals include Dulbecco’s Modified Eagle’s Medium (DMEM), streptomycin, penicillin, phosphate-buffered saline (PBS), trypsin/EDTA solutions, newborn calf serum (NBCS), sodium azide (NaN₃), β–nicotinamide adenine dinucleotide phosphate (β-NADPH), GSH, GSSG, NAC, BSO, N-ethylmaleimide (NEM), o-phtaldialdehyde (OPT), t-octylphenoxypolyethoxyethanol (Triton-X100), 1-chloro-2,4-dinitrobenzene (CDNB), tris hydroxymethyl aminomethane (Tris), ethylenediaminetetraacetic acid (EDTA), and H₂O₂ were purchased from Sigma-Aldrich (St. Louis, MO, USA). Methanol (MeOH) was acquired from Merck Life Science S.L. (Madrid, Spain). A Milli-Q water purification system (Millipore, Bedford, Burlington, MA, USA) was used to produce deionized water (resistivity < 18 MΩ cm). Standards of T-2 (MW: 466.52 g/mol), Neosolaniol (Neo; MW: 382.40 g/mol), T-2 triol (MW: 382.45 g/mol), and T-2 tetraol (MW: 298.33 g/mol) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Stock solutions of the mycotoxins were made in MeOH at the proper working concentrations and kept at a constant temperature of −20 °C in the dark.

Poly(lactide-co-glycolide) (PLGA, La:Ga ratio 85:15) was synthesized in Polish Academy of Sciences, Zabrze, Poland. Gentamycin sulfate, dioctyl sulfosuccinate sodium salt (AOT), gellan gum (GG, GelzanTM, MW 200–300 kDa), poly(vinyl alcohol) (PVA, Mowiol 4–88), o-phtaldialdehyde, 2-merkaptoethanol, calcein-AM, propidium iodide, resazurin, potassium bromide (KBr), and calcium chloride (CaCL₂) came from Sigma-Aldrich (Steinheim, Germany). Dichloromethane (DCM) and methanol were provided by Chemland (Stargard, Poland). Acetic acid and sodium acetate for buffer solution came from POCH (Gliwice, Poland). Modified Eagle Medium (MEM) used for the cell culture was upplemented with 10% fetal bovine serum (FBS) and 1% of the mixture of penicillin and streptomycin (all chemicals from PAN Biotech, Aidenbach, Germany). Phosphate-buffered saline concentrate (PBS buffer) was provided by VWR Life Science (Radnor, PA, USA).
The osteoblast-like cell line MC3T3-E1 (ATCC CRL-2593). The bacteria were from the reference strain of methicillin-resistant Staphylococcus aureus (MRSA, ATCC BAA-1681) and were cultured in trypticasein soy broth (TSB, BioMaxima S.A., Lublin, Poland).
The reference strain used in the study was methicillin-resistant Staphylococcus aureus ATCC^® BAA-1681™ (MRSA, American Type Culture Collection, Manassas, VA, USA).