Tissue proteins were extracted in RIPA buffer supplemented with complete Mini-Protease Cocktail (ROCHE). Primary antibodies against Sbp2, Gpx3, Dio1, and Txnrd2 (all from Proteintech) as well as Selenop (Santa Cruz) were used to quantify select Selenoprotein expression by immunoblotting after separation by SDS-PAGE; β-actin (Cell Signaling) was used as the internal protein loading control. Clarity ECL (Bio-Rad) was used for chemiluminescent detection of protein bands with band intensity quantified using Image J software.
Selenop
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Selenop is a laboratory product manufactured by Santa Cruz Biotechnology. It is a protein involved in the metabolism of selenium, an essential trace mineral. The core function of Selenop is to facilitate the transport and distribution of selenium in the body.
Automatically generated - may contain errors
Lab products found in correlation
Clarity ecl, by Bio-Rad (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Complete mini protease cocktail, by Roche (1 mentions)
P akt, by Cell Signaling Technology (1 mentions)
Pepck, by Santa Cruz Biotechnology (1 mentions)
Glut4, by Santa Cruz Biotechnology (1 mentions)
Primary antibodies against β actin, by Cell Signaling Technology (1 mentions)
P pi3k, by Cell Signaling Technology (1 mentions)
2 protocols using selenop
1
Quantification of Selenoprotein Expression
2
Investigating EGCG's Effects on Insulin Signaling
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EGCG (>99% purity) was purchased from Yibeijia Tea Technology, Inc. (Hangzhou, P.R. China). ACE and ACE2 activity assay kits were bought from BioVision Inc. (San Francisco, CA, USA). The primary antibodies against G6Pase-α, PEPCK, SELENOP, and GLUT4 were purchased from Santa Cruz Biotechnology (Dallas, TX, USA); primary antibodies against renin, AngII, ACE, ACE2, AT1R, AT2R, MasR, and Agt were obtained from Abcam (Cambridge, UK); primary antibodies against β-actin, TXNIP, PI3K, p-PI3K, AKT and p-AKT as well as anti-rabbit or anti-mouse immunoglobulin G as secondary antibodies, were purchased from Cell Signaling Technology, Inc. (Boston, MA, USA). ELISA kit for measurement of serum insulin was purchased from EMD Millipore Corporation, Billerica (San Diego, MA, USA). Rat thiredoxin reductase was obtained from Sigma (St. Louis, MO, USA). Other chemicals were of the highest grade available.
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