Simplemappr

All dissections performed for this study followed Lafontaine (2004) in methodology, with genitalia stored in glycerol-filled microcentrifuge vials. Genitalia of Cicinnus are incredibly intricate, complex, three dimensional structures, and therefore slide-mounting was not conducted in order to preserve the natural structural integrity. Labels of the holotype are given verbatim, with forward slashes used to denote separate labels.
Specimens examined are deposited in the collections listed below. Figures in this paper were created with Adobe Photoshop as part of the Creative Cloud (Adobe 2019), and maps were built using SimpleMappr (Shorthouse 2010 ). The following collections were used for specimens pertinent to the present study:
AMNHAmerican Museum of Natural History, New York, New York, USA;
BME Bohart Museum of Entomology, University of California, Davis, California, USA;
BWC B. Walsh Private Collection, Tucson, Arizona, USA;
CJM Collection of José Monzón, Guatemala;
CRAS Research collection of R. St Laurent, Gainesville, Florida, USA;
CUICCornell University Insect Collection, Ithaca, New York, USA;
MCZ Museum of Comparative Zoology, Harvard University, Cambridge, Massachusetts, USA;
MGCL McGuire Center for Lepidoptera & Biodiversity, Gainesville, Florida, USA;
PJD Collection of Paul J. Dennehy, Pennsylvania, USA;
VOB Becker Collection, Camacã, Bahia, Brazil;

Lamprochernes individuals were collected by compost sifting and extraction and from on or under tree bark across Europe (Table S1, Figure 1). The specimens were preliminarily determined, following the literature [56 (link)]. Distribution maps were created in SimpleMappr [71 ] and edited with Adobe Illustrator. Geographic distances among sample localities were obtained in Geographic Distance Matrix Generator [72 ]. To delimit Lamprochernes species, we first implemented a molecular data-based species discovery step, which was followed by a species validation step combining multispecies coalescent analyses (molecular data), morphometric analyses (morphology data) and cytogenetic analyses (karyotype data).

All 60 specimens studied for this paper were found among unsorted Microgastrinae in the

Canadian National Collection of Insects

(CNC).
Morphological terms and measurements of structures are mostly as in Mason (1981) , Huber and Sharkey (1993) , Whitfield (1997) , Karlsson and Ronquist (2012) , and Fernández-Triana et al. (2014). Mediotergites 1, 2, and so on, are abbreviated as T1, T2, etc.; ocular ocellar line as OOL and posterior ocellar line as POL.
Photos were taken using a Canon EOS 60D with MPE-65 lenses (aperture: 4.0, ISO: 100, CR2 format images) and a 600EX-RT Speedlight (manual) flash. Multiple images through the focal plane were taken of a structure, and these were combined to produce a single in-focus image with Zerene Stacker (http://zerenesystems.com/cms/stacker). Plates for the illustrations were prepared using Adobe Photoshop CS4.
A map with the distribution of all species was generated using SimpleMappr (Shorthouse 2010 ).