Immature MoDCs were co-cultivated with M. capsulatus Bath in 1:100 ratio (cells:bacteria) in medium free of antibiotics for 2–4 h in a humified atmosphere with 5% CO2. Cells were washed twice by phosphate buffered saline (PAA Laboratories), fixed with 4% PFA and 2.5% glutaraldehyde (1:1) for 20 min at room temperature. Cells were washed again, dehydrated in a graded ethanol series and dried using a critical point dehydrator (CPD030 BalTec). Samples were coated with ~500 Å Pt in a sputter coater (Polaron SC7640, Quorum technologies) and analyzed using an EVO-50 Zeiss microscope (Carl Zeiss AG).
Evo 50 microscope
Manufactured by Zeiss
Sourced in Germany, United States, United Kingdom
The EVO 50 is a scanning electron microscope (SEM) manufactured by Zeiss. It is designed to provide high-resolution imaging of samples at the micro and nano scale. The EVO 50 utilizes an electron beam to scan the surface of a sample, generating detailed images that can reveal the structure and composition of materials.
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Lab products found in correlation
8 protocols using evo 50 microscope
1
Visualizing Bacteria-Dendritic Cell Interaction
2
Scanning Electron Microscopy of Samples
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SEM was done as previously described (Christoffersen et al., 2015) . Samples were washed and fixed with 5% glutaraldehyde in 0.1 M sodium cacodylate and 0.1 M sucrose (pH 7.4) for 45 min; then replaced with 0.1 M sodium cacodylate and 0.1 M sucrose (pH 7.4) for 30 min.
Samples were then washed, dehydrated in graded ethanol series and dried using a critical-point dryer (CDP 030, BAL-TEC GmBH, Germany). Dry samples were mounted on aluminum stubs using double-faced carbon tape (Agar Scientific, UK), and coated with approximately 500 Å platinum using a sputter coater (Polaron SC7640, Quorum Technologies, UK). Microscopic analyses were performed using an EVO-50 Zeiss microscope (Carl Zeiss AG, Germany).
Samples were then washed, dehydrated in graded ethanol series and dried using a critical-point dryer (CDP 030, BAL-TEC GmBH, Germany). Dry samples were mounted on aluminum stubs using double-faced carbon tape (Agar Scientific, UK), and coated with approximately 500 Å platinum using a sputter coater (Polaron SC7640, Quorum Technologies, UK). Microscopic analyses were performed using an EVO-50 Zeiss microscope (Carl Zeiss AG, Germany).
3
Characterizing Modified Ti Surfaces
4
Multimodal Characterization of Samples
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SEM microscopy was done using an EVO50 microscope (ZEISS) with SE1 mode. TEM was performed on a JEOL 2000FX (JEOL) microscope. FTIR characterization was done on a Nicolet 6700 spectrometer. DSC measurements were done using a DSC822 (METTLER TOLEDO) thermal analyzer.
5
SEM Analysis of BNC Fiber Morphology
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Scanning electron microscopy (SEM) micrographs of the film surfaces were obtained on a Zeiss EVO 50 microscope (Zeiss, New York, NY, USA), equipped with a LaB6 electron gun and operating at 15 kV. SEM was used to measure BNC fibre diameters and to study their morphologies. Prior to the SEM observation, a few nanometers gold film was deposited onto the sample surface in order to avoid a charging effect during SEM testing. The BNC fibers diameters statistical distributions were obtained analyzing the SEM micrographs with ImageJ (version 1.52r, NIH, National Institutes of Health, Bethesda, MD, USA) software [26 (link)], collecting more than 300 diameter sizes for each specimen.
6
Characterization of Gold-Coated Particles
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The morphology
of gold-coated particles was investigated by scanning electron microscopy
(SEM) using a Zeiss-EVO 50 microscope under a 20 kV accelerating voltage.
The X-ray diffraction patterns were acquired using a Bruker-AXS D5005
diffractometer using Cu Kα radiation. The diffraction peaks
were indexed based on the databank of the Joint Committee on Powder
Diffraction Standard (JPDCS). The hydrodynamic diameter and zeta potential
(ζ) of the particles were measured using Zetasizer Nano ZS (Malvern
Instruments). For this, the particles were dispersed either in phosphate-buffered
solution (PBS, pH 7.4, 10 mM) or in a minimum essential medium (α-MEM,
Gibco) supplemented or not with 10% fetal bovine serum (FBS) and 1%
(v/v) penicillin/streptomycin. The measurements were carried out in
triplicate at room temperature.
of gold-coated particles was investigated by scanning electron microscopy
(SEM) using a Zeiss-EVO 50 microscope under a 20 kV accelerating voltage.
The X-ray diffraction patterns were acquired using a Bruker-AXS D5005
diffractometer using Cu Kα radiation. The diffraction peaks
were indexed based on the databank of the Joint Committee on Powder
Diffraction Standard (JPDCS). The hydrodynamic diameter and zeta potential
(ζ) of the particles were measured using Zetasizer Nano ZS (Malvern
Instruments). For this, the particles were dispersed either in phosphate-buffered
solution (PBS, pH 7.4, 10 mM) or in a minimum essential medium (α-MEM,
Gibco) supplemented or not with 10% fetal bovine serum (FBS) and 1%
(v/v) penicillin/streptomycin. The measurements were carried out in
triplicate at room temperature.
7
Microscopic Surface Examination Protocol
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An EVO 50 microscope (Zeiss, Cambridge, UK) was used to examine the surfaces. One specimen from each group was randomly selected. Prior to the scanning, the specimens were subjected to a metallization process (SCD 050, Sputter Coater Metals, Bal-Tec, Lichtenstein), in order to provide better contrast of the samples.
8
Analyzing Enamel Microstructure in Orai Mice
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