Isoliquiritigenin

Manufactured by Yuanye Bio-Technology

Sourced in China

Isoliquiritigenin is a pure chemical compound used for laboratory research and analysis. It is a flavonoid extracted from various plant sources. Isoliquiritigenin is utilized in the development and testing of analytical methods, as well as in studies investigating its potential biological activities.

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7 protocols using isoliquiritigenin

Isolation and Identification of Pinosylvin

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Pinosylvin was isolated in our laboratory from the root of Lindera reflexa Hemsl and identified using nuclear magnetic resonance (NMR), mass spectrometry (MS), ultraviolet (UV), and infrared (IR) analyses. Isoliquiritigenin (high-performance liquid chromatography [HPLC] ≥ 98%) was used as the internal standard and purchased from Shanghai Yuanye Bio-Technology Co. Ltd. Heparin sodium was purchased from Beijing Dingguo Changsheng Bio-Technology Co. Ltd. Methanol, acetonitrile, and formic acid were HPLC-grade reagents from Fisher Scientific (Fairlawn, NJ, USA). Deionized water was prepared by passing distilled water through a Milli-Q water purification system (Millipore, Milford, MA, USA).

Fu Y., Sun X., Wang L, & Chen S. (2018). Pharmacokinetics and Tissue Distribution Study of Pinosylvin in Rats by Ultra-High-Performance Liquid Chromatography Coupled with Linear Trap Quadrupole Orbitrap Mass Spectrometry. Evidence-based Complementary and Alternative Medicine : eCAM, 2018, 4181084.

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Characterization of Botanical Compounds

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Chlorogenic acid, neoChlorogenic acid, cryptoChlorogenic acid, arctiin, arctigenin, tectoridin, tectorigenin, iridin, irisflorentin, irigenin, matrine, oxymatrine, liquiritin, isoliquiritin, liquiritigenin, isoliquiritigenin, and galuteolin were obtained from the Shanghai YuanYe Biotechnology Co., Ltd. (Shanghai, China) for use as references. Glycyrrhizic acid and glycyrrhetinic acid were purchased from China’s National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). The purity of the reference compounds was >98% based on high-performance liquid chromatography (HPLC) analysis. SJLYKL was prepared by the Shanghai Liantang pharmacy (Shanghai, China). Deionized water was purified using a Milli-Q system (Millipore, Bedford, MA, USA). Ammonium acetate (HPLC grade; purity ≥98.0%) and acetic acid (HPLC grade; purity ≥99.7%) were provided by ANPEL Laboratory Technologies (Shanghai, China). Chromatographic grade methanol and acetonitrile were purchased from Merck (Darmstadt, Germany).

Gu J., Wu W., Huang M., Long F., Liu X, & Zhu Y. (2018). Application of High-Performance Liquid Chromatography Coupled with Linear Ion Trap Quadrupole Orbitrap Mass Spectrometry for Qualitative and Quantitative Assessment of Shejin-Liyan Granule Supplements. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(4), 884.

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Analytical Protocol for Botanical Compounds

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Linarin (internal standard, IS), harpagide, chlorogenic acid, swertiamarin, sweroside, angoroside C, liquiritin, and isoliquiritigenin (purity ≥98%) were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Methanol (HPLC grade) was purchased from Fisher Scientific (USA). Formic acid (MS grade) was purchased from ANPEL Laboratory Technologies Co., Ltd. (Shanghai, China). The distilled water was purchased from A.S. Watson Group Ltd. (Beijing, China).
Lonicera japonica Thunb. (JYH), Scrophularia ningpoensis Hems. (XS), Angelica sinensis (Oliv.) Diels (DG), and Glycyrrhiza uralensis Fisch. (GC) were purchased from the pharmacy of Dongzhimen Hospital of Beijing University of Traditional Chinese Medicine (Beijing, China).

Sun K.H., Yang M.F., Xu X.R., Li Y., Gao Z., Zhang Q.Y., Li H., Wang S.Q., Lou L.X., Wu A.M., Jin Q.S., Wu S.X, & Nie B. (2022). Comparative Pharmacokinetics of Seven Major Compounds in Normal and Atherosclerosis Mice after Oral Administration of Simiao Yong'an Decoction. Evidence-based Complementary and Alternative Medicine : eCAM, 2022, 4604601.

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Developmental Stages of Ougan Citrus Fruit

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Ougan (Citrus reticulata cv. Suavissima) tissues including roots, stems, leaves, flowers and fruits were obtained from Wenzhou, China. Fruits were harvested at eight different developmental stages, S1 (30 days after flower blooming, DAFB), S2 (60 DAFB), S3 (80 DAFB), S4 (100 DAFB), S5 (120 DAFB), S6 (140 DAFB), S7 (170 DAFB), S8 (200 DAFB), and the peels were separated from fruit, frozen in liquid nitrogen and stored at −80 °C until analysed. Three biological replicates were performed for analyses at each sampling point.
Naringenin, malonyl‐CoA and other flavonoid standards for measurement were purchased from Sigma‐Aldrich (https://www.sigmaaldrich.com). Naringenin chalcone, iso liquiritigenin, liquiritigenin and p‐coumaroyl‐CoA were purchased from Yuanye (http://www.shyuanye.com). All the chemicals were of HPLC (representative high‐performance liquid chromatography) grade.

Zhao C., Liu X., Gong Q., Cao J., Shen W., Yin X., Grierson D., Zhang B., Xu C., Li X., Chen K, & Sun C. (2020). Three AP2/ERF family members modulate flavonoid synthesis by regulating type IV chalcone isomerase in citrus. Plant Biotechnology Journal, 19(4), 671-688.

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Characterization of Wild Licorice Roots

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Wild licorice (all G. uralensis Fisch.) was collected from an arid hillside in Suide County, Shaanxi Province, transported on ice and stored at −80°C. It was identified by Professor Chunsheng Liu of Beijing University of Chinese Medicine. Numbers W-1, W-2, and W-3 were three groups of licorice with a thickness of 0.8, 0.9, and 1.1cm, and each group was composed of five licorice roots of the same thickness. The samples were all mixed with five licorice roots to form one sample. The reference substances liquiritin (Lot number: Z13J11X108109), glycyrrhizic acid (Lot number: P24J10F91300), iso liquiritigenin (Lot number: C03A8Q41092), liquiritigenin (Lot number: Z20J8X40265), and glycyrrhetinic acid (Lot number: T27J7X18416) were purchased from Shanghai Yuanye Bio Technology Co., Ltd. (Shanghai, China).

Li T., Ren G., Jiang D, & Liu C. (2021). Dynamic Changes in Endophytic Microorganisms and Metabolites During Natural Drying of Licorice. Frontiers in Microbiology, 12, 740721.

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Characterization of Zhi-Zi-Hou-Pu Extract

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Reference standards including agarotetrol, isoferulic acid, and harpagide were obtained from National Institutes for Food and Drug Control (Beijing, China). Liquiritigenin, isoLiquiritigenin, angoroside C, cimicifugine, glycyrrhizic acid, liquiritin, liquiritin apioside, isoliquiritin, trans-cinnamic acid, harpagoside, sucrose, formononetin, and licochalcone A were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). The purities of all the reference substances were higher than 98 %.
ZP samples were provided by Tianjin Hongrentang Pharmaceutical Co., Ltd. (Tianjin, China). LC-MS grade methanol was purchased from Sigma-Aldrich (St. Louis, MO, USA). Formic acid was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Water used for UPLC-Q-TOF-MS analysis was purified by Milli-Q water purification system (Millipore, Billerica, MA, USA).

Song L., Xu J., Shi Y., Zhao H., Zhang M., Wang Y., Cui Y, & Chai X. (2023). An integrated strategy of UPLC-Q-TOF-MS analysis, network pharmacology, and molecular docking to explore the chemical constituents and mechanism of Zixue Powder against febrile seizures. Heliyon, 10(1), e23865.

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Cultivation of Medicinal Plant Seeds

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After sterilization with 70% ethanol, tobacco (Nicotiana tabacum) seeds were sown on soil made up of a 3:1:1 combination of peat moss, perlite, and vermiculite (v/v/v). A fluorescent light cycle of 16/8 hours and a temperature of 26°C were used to cultivate seedlings. Seeds of soybean (Glycine max), Sophora flavescens, Glycyrrhiza uralensis, and Humulus lupulus were cultivated under the same condition. The standard compounds isobavachalcone (IBC) (CAS: 20784-50-3) and isoliquiritigenin (CAS: 961-29-5) were purchased from ShanghaiyuanyeBio-Technology Co., Ltd. (Shanghai, China).

Guo L., Zhao W., Wang Y., Yang Y., Wei C., Guo J., Dai J., Hirai M.Y., Bao A., Yang Z., Chen H, & Li Y. (2022). Heterologous biosynthesis of isobavachalcone in tobacco based on in planta screening of prenyltransferases. Frontiers in Plant Science, 13, 1034625.

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