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Immunocap phadia 100 laboratory system

Manufactured by Thermo Fisher Scientific
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Sourced in United States

The ImmunoCAP Phadia® 100 Laboratory System is a diagnostic instrument designed for in vitro testing of allergen-specific immunoglobulin E (IgE) levels in patient samples. The system utilizes fluorescent enzyme immunoassay technology to quantify IgE antibodies, providing clinicians with information to support the diagnosis of allergic conditions.

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Lab products found in correlation

Vacutainer serum tube, by BD (1 mentions)

Close spelling variants of this product

Phadia ImmunoCAP system Phadia 100 ImmunoCAP 100 ImmunoCAP system ImmunoCAP

2 protocols using immunocap phadia 100 laboratory system

1

Serum Immunoglobulin Quantification

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A sample of peripheral blood was taken into a BD Vacutainer™ serum tube (BD, Franklin Lakes, NJ, USA), allowing the blood to clot for ~20 min. Then, the serum was obtained by centrifugation at 130 g × 10 min and stored at −20 °C until tIgE and sIgE determination by fluoroenzymo immuno assay with the ImmunoCAP Phadia® 100 Laboratory System (Thermo Fisher Scientific Inc., Portage, MI, USA).
Salazar A., Casanova-Méndez I., Pacheco-Quito M., Velázquez-Soto H., Ayala-Balboa J., Graue-Hernández E.O., Serafín-López J, & Jiménez-Martínez M.C. (2019). Low Expression of IL-10 in Circulating Bregs and Inverted IL-10/TNF-α Ratio in Tears of Patients with Perennial Allergic Conjunctivitis: A Preliminary Study. International Journal of Molecular Sciences, 20(5), 1035.
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2

Serum IgE Measurement by FEIA

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The serum samples were processed to determine IgE by fluoro-enzyme-immunoassay (FEIA) in an ImmunoCAP Phadia® 100 Laboratory System (Thermo Fisher Scientific Inc., MI, USA), following the manufacturer’s instructions. The results were analyzed by the ImmunoCAP® software, v.4.13 (Thermo Fisher Scientific Inc., MI, USA), and the limit of detection was < 2 kUI/L.
Nieto J.E., Casanova I., Serna-Ojeda J.C., Graue-Hernández E.O., Quintana G., Salazar A, & Jiménez-Martinez M.C. (2020). Increased Expression of TLR4 in Circulating CD4+T Cells in Patients with Allergic Conjunctivitis and In Vitro Attenuation of Th2 Inflammatory Response by Alpha-MSH. International Journal of Molecular Sciences, 21(21), 7861.
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