Vitamin C (VC) tablets were purchased from Sanjing Pharmaceutical Co. Ltd. (Harbin, China). Trolox, fluorescein disodium salt and 2,2′-azobis (2-amidinopropane) dihydrochloride (ABAP) were purchased from Sigma-Aldrich (St. Louis, MO, USA). A mitochondria extraction kit and mitochondrial membrane potential assay kits were purchased from Beyotime Institute of Biotechnology (Guangzhou, China). Janus Green B (JC-B), a mitochondrial ROS fluorescence detection assay kit, a mitochondrial respiratory chain complex I kit and a mitochondrial respiratory chain complex II kit were all purchased from Shanghai GenMed Scientifics Inc. (Shanghai, China). Goat anti-rabbit immunoglobulin G (IgG) and an electrochemiluminescence (ECL) kit were purchased from Multisciences (Hangzhou, China). Rabbit anti-inducible iNOS (inducible nitric oxide synthase) antibody was purchased from Cell Signaling Technology Inc. (Boston, USA). The anti-mouse β-actin (1:2000) antibody was purchased from Santa Cruz Biotechnology Inc. (Dallas, Texas, USA). All other reagents were made in China and were analytically pure.
Goat anti rabbit immunoglobulin g igg
Manufactured by MultiSciences Biotech
Sourced in China
Goat anti-rabbit immunoglobulin G (IgG) is a secondary antibody produced in goats that specifically binds to rabbit immunoglobulin G (IgG). It is commonly used in various immunoassays and detection techniques to amplify and visualize the signal from primary antibodies raised in rabbits.
Automatically generated - may contain errors
Lab products found in correlation
Fluorescein disodium salt, by Merck Group (1 mentions)
Mitochondria extraction kit, by Beyotime (1 mentions)
Mitochondrial membrane potential assay kit, by Beyotime (1 mentions)
2 2 azobis 2 amidinopropane dihydrochloride, by Merck Group (1 mentions)
Trolox, by Merck Group (1 mentions)
Bicinchoninic acid disodium kit, by Beyotime (1 mentions)
Image pro plus software, by Media Cybernetics (1 mentions)
Gapdh, by MultiSciences Biotech (1 mentions)
2 protocols using goat anti rabbit immunoglobulin g igg
1
Mitochondrial Dysfunction Evaluation
2
Western Blot Analysis of PIWIL1 Protein
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Protein was extracted from tissues or cells using protein lysis buffer, and protein concentration was measured using the bicinchoninic acid disodium kit (Beyotime, Shanghai, China). Equivalent amounts of cell lysate were electrophoresed on a 10% SDS-PAGE gel and transferred onto polyvinylidene fluoride membranes. The membranes were sealed and incubated with primary antibody (rabbit anti-human PIWIL1 antibody, 1:500; Abcam, Cambridge, UK; PBS was used instead of the primary antibody as a negative control). GAPDH (Multisciences, Shanghai, China) was used as the internal reference. Subsequently, the membranes were incubated with secondary antibody goat anti-rabbit immunoglobulin G (IgG, 1:1,000; Multisciences). The band intensity was analyzed using Image-Pro Plus software (Version 6.0, Media Cybernetics, Inc., Rockville, MD, USA).
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