Log In Sign up
The largest database of trusted experimental protocols

Dab staining kit

Manufactured by Thermo Fisher Scientific
Visit Supplier

The DAB staining kit is a laboratory product designed for immunohistochemical detection. It provides a chromogenic substrate for the visualization of target proteins in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Cleaved caspase 3 asp175 5a1e, by Cell Signaling Technology (2 mentions) Anti ki67 sp6, by Thermo Fisher Scientific (2 mentions) S1699 retrieval buffer, by Agilent Technologies (2 mentions) Mouse human fcr blocker, by Miltenyi Biotec (2 mentions)

2 protocols using dab staining kit

1

Immunohistochemistry of Apoptosis Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Deparaffinized and rehydrated formalin fixed samples tumor samples were subjected to antigen retrieval using Dako S1699 Retrieval Buffer at pH 6.1 at 90oC for 30 mins. After a 5 min peroxidase blockade, slides were blocked for 10 min at RT with PBS containing 0.5% BSA, 5% goat serum, and 1X mouse/human FcR blocker (Miltenyi Biotec 130–092-575). Slides were incubated with primary anti-Bnip3 (EPR4034, Abcam) at 1:100 at RT for 30 min, rabbit mAb to cleaved Caspase-3 (Asp175) (5A1E) (Cell Signaling Technology) or anti-Ki67 (SP6) (Thermo-Scientific) at 1:100 overnight at 4oC. IHC was visualized by DAB staining kit (TL-015-HD by Invitrogen), counterstained with hematoxylin, and mounted in non-aqueous solution (Richard-Allen Scientific 4112).
Nollet E.A., Cardo-Vila M., Ganguly S.S., Tran J.D., Schulz V.V., Cress A., Corey E, & Miranti C.K. (2020). Androgen Receptor-Induced Integrin α6β1 and Bnip3 Promotes Survival and Resistance to PI3K Inhibitors in Castration-Resistant Prostate Cancer. Oncogene, 39(31), 5390-5404.
+ Open protocol
+ Expand
2

Immunohistochemistry of Apoptosis Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Deparaffinized and rehydrated formalin fixed samples tumor samples were subjected to antigen retrieval using Dako S1699 Retrieval Buffer at pH 6.1 at 90oC for 30 mins. After a 5 min peroxidase blockade, slides were blocked for 10 min at RT with PBS containing 0.5% BSA, 5% goat serum, and 1X mouse/human FcR blocker (Miltenyi Biotec 130–092-575). Slides were incubated with primary anti-Bnip3 (EPR4034, Abcam) at 1:100 at RT for 30 min, rabbit mAb to cleaved Caspase-3 (Asp175) (5A1E) (Cell Signaling Technology) or anti-Ki67 (SP6) (Thermo-Scientific) at 1:100 overnight at 4oC. IHC was visualized by DAB staining kit (TL-015-HD by Invitrogen), counterstained with hematoxylin, and mounted in non-aqueous solution (Richard-Allen Scientific 4112).
Nollet E.A., Cardo-Vila M., Ganguly S.S., Tran J.D., Schulz V.V., Cress A., Corey E, & Miranti C.K. (2020). Androgen Receptor-Induced Integrin α6β1 and Bnip3 Promotes Survival and Resistance to PI3K Inhibitors in Castration-Resistant Prostate Cancer. Oncogene, 39(31), 5390-5404.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!