Animals were euthanized when moribund as per Stanford Animal Welfare protocol guidelines, or at 100 days after transplantation if they survived without morbidity. Histopathological specimens were obtained from lungs and livers of hosts. Tissues were fixed in 10% formalin, stained with hematoxylin and eosin and images were obtained using an Eclipse E1000M microscope (Nikon, Melville, NY, USA).
Eclipse e1000m microscope
Manufactured by Nikon
Sourced in United States
The Eclipse E1000M microscope is a high-quality optical instrument designed for advanced laboratory applications. It features a range of technical specifications, including a high-resolution optical system, ergonomic design, and versatile functionality. The Eclipse E1000M is intended for use in various scientific research and analysis settings, providing users with reliable and precise imaging capabilities.
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Lab products found in correlation
4 protocols using eclipse e1000m microscope
1
Histopathological Tissue Analysis
2
Heparin and Chondroitin Sulfate Modulate Caudal Fin Regeneration
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At 3 dpa, Tg(Fli1:EGFP) fish were anesthetized and 100 μg/mL unfractionated heparin from porcine skin (Sigma), 1% phenol red in 1x PBS, or 100 μg/mL chondroitin sulfate (Sigma) was injected into the third and fourth fin rays of the dorsal regenerating caudal fin. The uninjected ventral fin rays served as an internal control. After 24 h (4 dpa), fins were imaged using a Nikon Eclipse 80i microscope with a 4x objective and a Nikon Eclipse E1000M microscope with 1x or 4x objectives. Brightfield and EGFP fluorescence (FITC filter) images were obtained for both injected and uninjected sides of each fin. The fins were then harvested as the MO-injected fins and processed for vascular phenotype, total and vasculature regenerate length, and cell proliferation analyses.
3
Histopathological Analysis of Tumor Samples
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Tumors from both flanks, as well as lung tissue in applicable cases, were extracted and fixed in 10% formalin. Sections were stained with hematoxylin and eosin (H&E), and images were obtained using an Eclipse E1000M microscope (Nikon). For CD8 immunohistochemistry, paraffin-embedded tumor tissue was sliced into 5 μm-thick sections with a microtome, air-dried, fixed with acetone, and stained by the DFCI Rodent Histopathology Core. Immunostaining was performed using anti-CD8 (Abcam) according to the manufacturer's protocol. Multi-color images were obtained using a Zeiss fluorescent microscope.
4
Complement Activation Quantification
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Commercially available enzyme immune assay (Complement System Screen Wieslab; Euro Diagnostica, Malmö, Sweden) and murine anti-human C5b-9 antibody (clone aE11, Dako, Glostrup, Denmark) were used according to manufacturer’s instructions to detect functional complement activity and sC5b-9 production in plasma, respectively. Both methods detect the respective human and pig epitopes [41 (link)]. In tissue, the membrane form of C5b-9 was visualized in frozen sections from the AAR, border zone and control area. Tissue samples were incubated for 30 min at room temperature using the murine anti-human C5b-9 antibody (clone aE11, Dako, Glostrup, Denmark) diluted 1/25 in Dako antibody diluent (Dako K8006, Glostrup, Denmark), washed in phosphate buffered saline and stained by Ventana ultra View Universal DAB Detection Kit (Ventana Medical Systems, Inc., Tucson, AZ) according to the manufacturer’s instructions. A Nikon Eclipse E1000M microscope was used and photos were obtained with original 40× magnification.
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