Recombinant human ccl2

Manufactured by BioLegend

Recombinant human CCL2 is a cytokine that belongs to the CC chemokine family. It is involved in the recruitment and activation of monocytes, macrophages, and other immune cells.

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Lab products found in correlation

Ab21667, by Abcam (1 mentions) Ab120812, by Abcam (1 mentions) Ccl22, by R&D Systems (1 mentions) Ab83250, by Abcam (1 mentions) Anti akt antibody 9272, by Cell Signaling Technology (1 mentions) Azd5363 s8019, by Selleck Chemicals (1 mentions) Anti gapdh antibody nb300 221, by Novus Biologicals (1 mentions) Stat3 inhibitor s3i 201, by Selleck Chemicals (1 mentions)

2 protocols using recombinant human ccl2

Evaluating Chemokine Receptor Signaling Pathways

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Recombinant human CCL17 and CCL22 were purchased from R&D Systems (Minneapolis, MN). Recombinant human CCL2 was obtained from BioLegend (San Diego, CA). Antibodies used in western blotting, IHC, immunocytochemistry, and flow cytometry were as follows: rabbit anti-human CCR2 antibody (ab21667), rabbit anti-human CCR4 antibody (ab83250), goat anti-human CCR4 antibody (ab1669), rabbit anti-goat immunoglobulin (Ig)G H&L (fluorescein isothiocyanate; FITC) antibody (6737), goat anti-rabbit IgG H&L (FITC) antibody (6717), anti-mannose receptor antibody (ab64693), and anti-CCR7 antibody (ab103404) obtained from Abcam (Cambridge, MA); anti-Akt antibody (9272), anti-phospho-Akt (Thr308) antibody (9275), anti-phospho-Akt (Ser473) antibody (9271), and anti-rabbit IgG HRP-linked antibody (7074) obtained from Cell Signaling Technology (Danvers, MA); and anti-GAPDH antibody (NB300-221) obtained from Novus Biologicals (Littleton, CO). The antagonists used in the neutralizing assay were a selective CCR2 antagonist (ab120812) obtained from Abcam and a CCR4 antagonist (SC-221406) obtained from Santa Cruz Biotechnology (Dallas, TX). The Akt inhibitor AZD5363 (S8019) was obtained from Selleckchem (Houston, TX).

Maolake A., Izumi K., Shigehara K., Natsagdorj A., Iwamoto H., Kadomoto S., Takezawa Y., Machioka K., Narimoto K., Namiki M., Lin W.J., Wufuer G, & Mizokami A. (2016). Tumor-associated macrophages promote prostate cancer migration through activation of the CCL22–CCR4 axis. Oncotarget, 8(6), 9739-9751.

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Inhibiting Monocyte Random Migration

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Isolated human CD14⁺CD16⁻ monocytes were incubated with various inhibitors upon seeding. Several inhibitors were used to interfere with the random migration of monocytes: 20 μM RhoA Kinase ROCK inhibitor Y-27632 (Selleckchem), 100 μM Arp inhibitor CK666 (Selleckchem), 100 μM STAT3 inhibitor S3I-201 (Selleckchem), 10 μM Blebbistatin for non-muscle myosin II inhibition (Millipore Sigma), and 10 μM EIPA (Millipore Sigma) to block the sodium-hydrogen exchanger (NHE). Monocytes were incubated with 2μg/mL Pertussis toxin (EMD Millipore Crop), 20 μM CID-1067700 (MedChemExpress), and 2 μg/mL recombinant human CCL2 (Biolegend).

Wirtz D., Du W., Zhu J., Wu Y., Kiemen A., Wan Z., Hanna E, & Sun S. (2023). Mechano-induced homotypic patterned domain formation by monocytes. Research Square.

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