Tris 2 carboxyethyl phosphine hydrochloride tcep

Lipids, such as 1,2-distearoyl-sn-glycero-3-phosphatidylcholine (DSPC), N-(carbonyl-methoxypolyethyleneglycol2000)-1,2-distearoyl-sn-glycero-3 phosphatidyl-ethanolamine (DSPE-PEG2000), and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide(polyethylene glycol)-2000] (ammonium salt) (DSPE-PEG2000-Mal), were purchased from NOF Corporation (Tokyo, Japan). Tris(2-carboxyethyl)phosphine hydrochloride (TCEP) and L-cysteine were purchased from FUJIFILM Wako Pure Chemical Corporation (Tokyo, Japan). Herceptin^® (trastuzumab) was purchased from Chugai Pharmaceutical Co., Ltd. (Tokyo, Japan). 1,1-dioctadecyl-3,3,3,3-tetramethyl-indocarbocyanine perchlorate (DiI) was purchased from Setareh Biotech LLC (Eugene, OR, USA).

DPAEMA (97%) was obtained from Sigma-Aldrich (St. Louis, MO, USA) and purified using a Sigma-Aldrich inhibitor removal column. MPC was purchased from NOF Corp. (Tokyo, Japan), which was synthesized and purified following a previously reported method [28 (link)]. 4-Cyanopentanoic acid dithiobenzoate (CPD) was synthesized as a chain transfer agent (CTA) according to a reported procedure [29 (link)]. Tris(2-carboxyethyl)phosphine hydrochloride (TCEP, 98%), ethylenediamine (>99.0%), and 4,4′-azobis-(4-cyanovaleric acid) (V-501, 98%) were purchased from Wako Pure Chemical (Osaka, Japan) and used without any purification procedure. Pyrene (Py, 97%) was supplied by Wako Pure Chemical and purified via recrystallization from methanol. Alexa Fluor 555 C₂ maleimide (A555) and Alexa Fluor 488 C₅ maleimide (A488) were purchased from Thermo Fisher Scientific (Tokyo, Japan) and used as received. Methanol and ethanol were dried over 4Å molecular sieves and purified via distillation. Water was purified using an ion-exchange column.