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1 step slow tmb elisa substrate

Manufactured by Thermo Fisher Scientific
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The 1-Step Slow TMB ELISA Substrate is a ready-to-use, stable solution for the colorimetric detection of horseradish peroxidase (HRP) in enzyme-linked immunosorbent assay (ELISA) applications. The substrate solution contains 3,3',5,5'-tetramethylbenzidine (TMB) and hydrogen peroxide, which react in the presence of HRP to produce a blue color that can be measured spectrophotometrically.

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Il 10, by BD (1 mentions) Stop solution, by Thermo Fisher Scientific (1 mentions) Interleukin 6 (il 6), by BD (1 mentions) Tnf α, by BD (1 mentions) Ni nta hissorb strips, by Qiagen (1 mentions)

Close spelling variants of this product

1-Step Slow TMB-ELISA TMB-ELISA substrate TMB substrate ELISAs

2 protocols using 1 step slow tmb elisa substrate

1

Multiplex ELISA for Inflammatory Cytokines

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Plasma and cellular supernatants were measured using ELISA kits for MCP-1 (BD Biosciences, 555179), IL-6 (BD Biosciences, 555220), TNFα (BD Bioscience, 555212), IL-8 (BD Biosciences, 555244), MCP-2 (CCL8) (Biolegend, 442204), and IL-10 (BD Biosciences, 555157). 1-Step Slow TMB ELISA substrate (Thermo Scientific, 34024) and Stop Solution (Invitrogen, 5504) were used for colorimetric detection.
Eitas T.K., Stepp W., Sjeklocha L., Long C., Riley C., Callahan J., Sanchez Y., Gough P., Knowlin L., van Duin D., Ortiz-Pujols S., Jones S., Maile R., Hong Z., Berger S, & Cairns B. (2017). Differential regulation of innate immune cytokine production through pharmacological activation of Nuclear Factor-Erythroid-2-Related Factor 2 (NRF2) in burn patient immune cells and monocytes. PLoS ONE, 12(9), e0184164.
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2

Quantitative Strep-Tag Protein Assay

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ELISA was performed in Ni-NTA HisSorb Strips (Qiagen, Hilden, DE) according the manufacturer instructions. A 1:5,000 dilution of monoclonal anti-Strep-tag II antibodies conjugated to HRP (IBA, Goettingen, DE), the 1-Step Slow TMB-ELISA substrate and Stop Solution (Thermo Scientific, Waltham, MA, US) were used for colorometric detection at OD450nm after 30 min of incubation. All reactions were performed in triplicate and wells without Dip, without RNA degradosome or without both proteins were used as a negative control.
Van den Bossche A., Hardwick S.W., Ceyssens P.J., Hendrix H., Voet M., Dendooven T., Bandyra K.J., De Maeyer M., Aertsen A., Noben J.P., Luisi B.F, & Lavigne R. (2016). Structural elucidation of a novel mechanism for the bacteriophage-based inhibition of the RNA degradosome. eLife, 5, e16413.
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