Bronchoalveolar lavage fluid (BALF) samples (1 ml) were obtained from each mouse. Samples were centrifuged (600 g, 3 min), and the supernatant was stored at –20 °C for cytokine analysis. The cell pellets from samples were pooled for total cell counts using a Model Z1 (Beckman-Coulter, Miami, FL, USA) after lysis of erythrocytes (Zap-Oglobin II, Beckman-Coulter, Fullerton, CA, USA). Slides were loaded with cells, centrifuged (700 × g, 3 min) and stained with Diff-Quick (Baxter, Detroit, MI, USA). Differentials were enumerated via light microscopy.
Model z1
Manufactured by Beckman Coulter
Sourced in United States
The Model Z1 is a particle counter and sizer that measures the size and concentration of particles in a sample. It uses the Coulter Principle to detect and analyze particles suspended in an electrolyte solution.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using model z1
1
Bronchoalveolar Lavage Cytometry Analysis
2
Analysis of Bronchoalveolar Lavage Fluid
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BALF samples (1 ml) were obtained from each mouse and centrifuged (600× g for 3 min), and the supernatant was stored at −20°C for cytokine analysis. The cell pellets were pooled for determining total cell counts using a particle counter (Model Z1; Beckman‐Coulter, Miami, FL, USA) after lysing the erythrocytes (Zap‐Oglobin II; Beckman‐Coulter). Slides were loaded with cells, centrifuged (700× g for 3 min), and stained with Diff‐Quick (Baxter, Detroit, MI, USA). Lymphocytes, eosinophils, and neutrophils were counted under a light microscope.
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