Tacs2 tdt dab

Manufactured by Bio-Techne

The TACS2 TdT-DAB is a laboratory reagent produced by Bio-Techne. It is used for the detection of DNA fragmentation associated with apoptosis, a programmed cell death process. The product utilizes terminal deoxynucleotidyl transferase (TdT) to label DNA ends, which are then visualized using a chromogenic detection system.

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Lab products found in correlation

Rm 9106 s1, by Thermo Fisher Scientific (1 mentions) Dye binding protein assay, by Bio-Rad (1 mentions) Annexin 5 cy5 apoptosis detection kit, by Abcam (1 mentions)

4 protocols using tacs2 tdt dab

Lung Apoptosis Quantification by TUNEL

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Apoptotic cells in the lung were detected by TUNEL assay using the in situ apoptosis detection kit (TACS2 TdT-DAB; Trevigen, Gaithersburg, MD) according to the manufacturer’s instructions except that hematoxylin was used for counterstaining. The number of positive cells was counted under microscope.

Kawara A., Mizuta R., Fujisawa M., Ito T., Li C., Nakamura K., Sun C., Kuwabara M., Kitabatake M., Yoshimura T, & Matsukawa A. (2020). Spred2-deficiency enhances the proliferation of lung epithelial cells and alleviates pulmonary fibrosis induced by bleomycin. Scientific Reports, 10, 16490.

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Paraffin IHC Protocol for Cancer Research

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Paraffin-embedded tissue blocks were used for IHC in the Sidney Kimmel Cancer Center Pathology Core Facility as previously described (40 (link)). The antibody to Dach1 (10914-1-AP, Proteintech), Ki67 (RM-9106-S1, Thermo) and TUNEL (TACS2 TdT DAB, Trevigen) was previously described (28 (link)).

Chen K., Wu K., Jiao X., Wang L., Ju X., Wang M., Di Sante G., Xu S., Wang Q., Li K., Sun X., Xu C., Li Z., Casimiro M.C., Ertel A., Addya S., McCue P., Lisanti M.P., Wang C., Davis R.J., Mardon G, & Pestell R.G. (2015). The Endogenous Cell-Fate Factor Dachshund restrains Prostate Epithelial Cell Migration via Repression of Cytokine Secretion via a CXCL Signaling Module. Cancer research, 75(10), 1992-2004.

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Apoptosis and Caspase Activities in Liver

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Apoptotic cells in the liver were detected by TUNEL assay using an in situ apoptosis detection kit (TACS2 TdT-DAB; Trevigen) as per the manufacturer’s instructions and the positive cells were counted under a microscope. To measure caspase-3, caspase-8, and caspase-9 activities, a colorimetric assay kit (MBL, Nagoya, Japan) for each caspase was used. Livers were homogenized in the lysis buffer provided in the kit and the activities in cytosolic extracts (150 µg protein) were measured. Protein concentrations in the extracts were measured in a protein-dye binding assay (Bio-Rad Laboratories, Hercules, CA, USA).

Yang X., Fujisawa M., Yoshimura T., Ohara T., Sato M., Mino M., San T.H., Gao T., Kunkel S.L, & Matsukawa A. (2018). Spred2 Deficiency Exacerbates D-Galactosamine/Lipopolysaccharide -induced Acute Liver Injury in Mice via Increased Production of TNFα. Scientific Reports, 8, 188.

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Apoptosis Quantification in Tissues

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Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) (TACS2 TdT DAB, Trevigen) staining was used as a measure of apoptosis in tissues as previously described [85 (link)]. Apoptosis was quantified by Flow Cytometry using the Annexin V-Cy5 apoptosis detection kit, as per the manufacturer’s instructions (Abcam).

Pestell T.G., Jiao X., Kumar M., Peck A.R., Prisco M., Deng S., Li Z., Ertel A., Casimiro M.C., Ju X., Di Rocco A., Di Sante G., Katiyar S., Shupp A., Lisanti M.P., Jain P., Wu K., Rui H., Hooper D.C., Yu Z., Goldman A.R., Speicher D.W., Laury-Kleintop L, & Pestell R.G. (2017). Stromal cyclin D1 promotes heterotypic immune signaling and breast cancer growth. Oncotarget, 8(47), 81754-81775.

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