Male crimp terminals

Silver wires (100 μm diameter, Sigma) were soldered into male crimp-terminals (RS Components). The tips of the wires were incubated in sodium hypochlorite, NaClO (10% active chlorine, Sigma) for 1 h to form Ag/AgCl electrodes. The tips of the electrodes were coated with a hydrogel layer by pipetting melted agarose (1% low-melt agarose, Sigma) onto their surfaces. For single DIB experiments, each electrode was plugged into a female crimp-terminal, which was attached to a micromanipulator (Narishige, NMN-21). The other end of the female crimp-terminal (RS Components) was soldered to a cable that terminated with a male crimp, which was connected to the headstage of the amplifier.

Silver wires (100 μm diameter, Sigma) were soldered into male crimp-terminals (RS Components). The tips of the wires were incubated in sodium hypochlorite, NaClO (10% active chlorine, Sigma) for 45 min to form Ag/AgCl electrodes. The tips of the electrodes were coated with a hydrogel layer by pipetting melted agarose (2% low-melt agarose, Sigma) onto their surfaces. Each electrode was plugged into a female crimp-terminal, which was attached to a micromanipulator (Narishige, NMN-21). The other end of the female crimp-terminal (RS Components) was soldered to a cable that terminated with a male crimp, which was connected to the voltage-clamp amplifier (Triton+, Tecella LLC) via an electrode probe holder (Terrapin, Tecella LLC). The current signal was obtained by an episodic acquisition routine (0 mV, 50 mV, 0 mV, −50 mV, 0 mV, in 5 s intervals) with a feedback resistor of 1 GΩ. Data were acquired at 20 kHz with a 5 kHz filter through the ‘TecellaLab v0.90 type 2’ software. The data was in Tecella’s tlc binary file type and analysed and displayed by using custom software written in LabVIEW^{31 (link)}.