Galaxy r co2 chamber

When cultures reached to 60%, they were exposed to the experimental gas mixture which consisted of 21% O₂, 5% CO₂ and either 3.6% sevoflurane or 10.3% desflurane balanced with N₂ (BOC, South Humberside, UK) in a purpose-built 1.5 L airtight gas chamber, equipped with inlet and outlet valves. The chamber was placed in an incubator (Galaxy R CO₂ chamber; New Brunswick Scientific, Enfield, CT, USA) at 37 °C for 2 h [53 (link)]. Other cohort cells were exposed to the same concentration gases without inhalational anesthetic served as controls. After exposure, cells were returned to the normal culture incubator until the further study.

Cells were placed in a purpose-built 1.5L airtight gas chamber, equipped with inlet and outlet valves and an electric fan to ensure an even distribution of gases throughout the exposure period. All gases (BOC, South Humberside, UK) were delivered to the gas chamber at a rate of 2L/min for a maximum of 5mins until the desired gas and anaesthetic concentrations were achieved. The chamber gases were monitored using an anaesthetic analyser (Datex-Ohmeda, Stirling, UK) until the chamber was sealed. The chamber was then placed in an incubator (Galaxy R CO₂ chamber; New Brunswick Scientific, Enfield, USA) at 37°C for the duration of the 2 hour incubation. The experimental gas mixtures were 21% oxygen, 5% carbon dioxide and 2% isoflurane, 3.6% sevoflurane or 10.3% desflurane, balanced with nitrogen. The equal 1.7 minimum alveolar concentrations (MAC) in human of these volatile anaesthetic concentrations was used in this study. After exposure, cultures were returned to the normal culture incubator for further study.

When cultures reached 60%, they were exposed to 21% O₂, 5% CO₂ and 3.6% sevoflurane or 10.3% desflurane balanced with N₂ (BOC, South Humberside, UK) in a purpose-built 1.5 L airtight gas chamber, equipped with inlet and outlet valves. The chamber was then placed in an incubator (Galaxy R CO₂ chamber; New Brunswick Scientific, Enfield, CT, USA) at 37 °C for 2 h. Other cohort cells were exposed to the same concentration gases without any inhalational anaesthetics and served as controls. After exposure, cells were returned to the normal culture incubator for further study. Both sevoflurane and desflurane were set at the equipotent concentrations approximately equal to 1.7 minimum alveolar concentrations in human.