Slc25a46

Manufactured by Proteintech

SLC25A46 is a membrane protein that functions as a mitochondrial carrier. It is responsible for the transport of molecules across the mitochondrial inner membrane.

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Lab products found in correlation

Mid51, by Proteintech (1 mentions) Ubiquitin fk2, by Enzo Life Sciences (1 mentions) Mln4924, by Merck Group (1 mentions) Nms 873, by Selleck Chemicals (1 mentions) Ha ha 11, by BioLegend (1 mentions) Lc3a b, by Cell Signaling Technology (1 mentions) Mg132, by Enzo Life Sciences (1 mentions) Bafilomycin a1, by Merck Group (1 mentions) Myc 9e10, by Developmental Studies Hybridoma Bank (1 mentions) α tubulin, by Santa Cruz Biotechnology (1 mentions) Itpr1, by Cell Signaling Technology (1 mentions) Vdac1, by Abcam (1 mentions) Uqcrc2, by Abcam (1 mentions) P616 drp1, by Cell Signaling Technology (1 mentions) Cox 2, by Cell Signaling Technology (1 mentions) Cdkn1a p21, by Cell Signaling Technology (1 mentions) P mdm2, by Cell Signaling Technology (1 mentions) Ndufa9, by Abcam (1 mentions) Tomm20, by Santa Cruz Biotechnology (1 mentions) Lamp1, by Santa Cruz Biotechnology (1 mentions)

2 protocols using slc25a46

Organelle Protein Regulation Assay

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MG132 (Enzo Biochem), NMS873 (Selleck Chemicals), bortezomib, bafilomycin A1, and MLN4924 (EMD Millipore) were dissolved in DMSO, stored at −80°C, and used at the indicated concentrations. DMSO at 0.1% served as the vehicle control.
Antibodies used in this study were SLC25A46 (Proteintech); HA (HA.11; Biolegend); MFN1 (ab57602) and histidine (HIS.H8; Abcam); LC3A/B (Cell Signaling); MFN2 (D1E9; Cell Signaling); MTCH2 (Abgent); MIC60/IMMT (Abgent and Proteintech); MID51 (Proteintech); Ubiquitin (FK2, Enzo Biochem); Mortalin (University of California, Davis/National Institutes of Health NeuroMab Facility); MIC19/CHCHD3 (Everest Biotech); MIC27/Apool (Assay BioTech); OPA1, P97/VCP, and TIMM23 (BD Biosciences); TOMM20 (FL-145), LRP130 (H-300), and glyceraldehyde-3-phosphate dehydrogenase (FL-335; Santa Cruz Biotechnology); MULAN (HPA017681) and Flag (M2) (Sigma-Aldrich); MARCH5 (provided by Richard Youle); myc (9E10; Developmental Studies Hybridoma Bank, University of Iowa); and Cullin 4a (PA5-29857; Thermo Fisher Scientific). PREP, GFP, TOMM40, YME1L, and PNPase polyclonal antibodies were raised against recombinant proteins (Pacific Immunology).

Steffen J., Vashisht A.A., Wan J., Jen J.C., Claypool S.M., Wohlschlegel J.A, & Koehler C.M. (2017). Rapid degradation of mutant SLC25A46 by the ubiquitin-proteasome system results in MFN1/2-mediated hyperfusion of mitochondria. Molecular Biology of the Cell, 28(5), 600-612.

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Antibody-Based Mitochondrial Protein Analysis

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Antibodies directed against the following were used in this study: SLC25A46 (Proteintech, 12277‐1‐AP), MFN2 (Santa Cruz, sc‐100560), SDHA (Abcam, ab14715), AIFM1 (Chemicon, MABN92), ATP5A1 (Abcam, ab14748), HCCS (Proteintech, 15118‐1‐AP), SCO1 (in‐house), MRPL14 (Proteintech, 15040‐1‐AP), NDUFA9 (Abcam, ab14713), UQCRC2 (Abcam, ab14745), COX4 (in‐house), COX2 (in‐house), MFN1 (Cell Signaling, 14739), IMMT/MIC60 (Abnova, H00010989‐M01), CHCHD3/MIC19 (Aviva, ARP57040‐P050), OPA1 (BD biosciences, 612607), MFF (Sigma, SAB1305258), CDKN1A/p21 (Cell Signaling, 2946), p53 (Abcam, ab176243), p‐Mdm2 (Cell Signaling, 3521), LAMP1 (Santa Cruz, sc‐18821), VIM (BD biosciences, 550513), VDAC1 (Abcam, ab14734), EMC1 (GeneTex, GTX119884), EMC2 (ProteinTech, 25443‐1‐AP) and FlagM2 (Sigma, F1804), TOMM20 (Santa Cruz, sc‐11415), DRP1 (BD biosciences, 611113), p637‐DRP1 (Cell Signaling, 6319), p616‐DRP1 (Cell Signaling, 3455), KDEL (Abcam, ab50601), ITPR1 (Cell Signaling, 8568), alpha‐tubulin (Santa Cruz, sc‐23948).

Janer A., Prudent J., Paupe V., Fahiminiya S., Majewski J., Sgarioto N., Des Rosiers C., Forest A., Lin Z., Gingras A., Mitchell G., McBride H.M, & Shoubridge E.A. (2016). SLC25A46 is required for mitochondrial lipid homeostasis and cristae maintenance and is responsible for Leigh syndrome. EMBO Molecular Medicine, 8(9), 1019-1038.

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