Prepfiler bta forensic dna extraction kit

After extracting human genomic DNA from 286 bloodstain samples using PrepFiler BTA™ Forensic DNA Extraction kit (Thermo Fisher Scientific, MA, USA), a multiplex PCR amplification was performed with the selfdeveloped 6-dye 64-plex panel on the GeneAmp PCR System 9700 Thermal Cycler (Applied Biosystems, Foster City, CA, USA) according to the previously reported research. And then the PCR products were removed from the PCR cycler and then genotyped by capillary electrophoresis on an ABI 3500xL Genetic Analyzer (Applied Biosystems, Foster City, USA). The DNA profiles of the 64-plex panel were visualized and analyzed by GeneMapper ID-X software version 1.5 (Applied Biosystem, Foster City, USA).

The DNA from mock crime scene samples was purified using PrepFiler Forensic DNA Extraction Kit (Thermo Fisher Scientific) for samples on cotton-tipped swabs (Puritan Medical Products Company LLC, Guilford, ME) and PrepFiler BTA Forensic DNA Extraction Kit (Thermo Fisher Scientific) for chewing gum and cigarette butt paper samples by following manufacturer's recommendation. For all samples, 50 μL of PrepFiler Elution Buffer was used.