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Pser10 histone h3

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The PSer10 histone H3 is a laboratory tool used in life science research. It is a post-translationally modified histone H3 protein with a phosphorylated serine residue at position 10. This modification is often associated with various cellular processes, such as chromatin remodeling and gene regulation. The PSer10 histone H3 can be used as a research reagent in techniques like Western blotting, immunoprecipitation, and chromatin immunoprecipitation to study the role of this specific histone modification in different biological systems.

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Lab products found in correlation

Okadaic acid, by Merck Group (2 mentions) Ap20545pu n, by Santa Cruz Biotechnology (2 mentions) Irdye800 conjugated anti mouse and anti rabbit, by Rockland Immunochemicals (2 mentions) Forskolin, by Merck Group (2 mentions) Myc tag, by Merck Group (2 mentions) Tautomycetin, by Bio-Techne (2 mentions) β actin, by Merck Group (2 mentions) Anti mouse cy3 conjugated antibody, by Thermo Fisher Scientific (1 mentions) Tetradecanoyl phorbol acetate, by Merck Group (1 mentions) Caffeine, by Merck Group (1 mentions)

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Histone H3 (77 citations) Histones (7 citations)

2 protocols using pser10 histone h3

1

Phosphorylation and Regulation of Neuronal Proteins

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Primary antibodies were: mouse monoclonal for DARPP-32 5a and 6 (ref. 58 (link); 1/5,000), calmodulin (Upstate Cell Signaling # 05–173); rabbit polyclonal for pThr34, pThr75 and pSer97 (#12438, #2301, #3401; 1:2,000; Cell Signaling); total β-adducin (#AP20545PU-N, 1:500; Santa Cruz, Acris); pSer713 of β-adducin (also reacts with pSer662 of γ-adducin and pSer724 of α-adducin; #05-587; 1:1,000; Chemicon/Millipore); β-actin (#A5316; 1:1,000; Sigma); myc-tag (#05-724; 1:500; Millipore); GFP (Abcam #ab6556, 1/1,000); pThr79-pSer81-calmodulin (Abcam, #ab194526); and pSer10 histone H3 (#06-570; rabbit; Millipore). Secondary antibodies comprised of IRDye800-conjugated anti-mouse and anti-rabbit (#610-132-121, #611-132-122; both 1:4,000; Rockland) antibodies for immunoblotting and anti-mouse CY3-conjugated antibody (#A10521; 1:400; Molecular Probes) for immunochemistry. COS-7 cells were treated with one or the combination of the following compounds, as indicated, or with dimethyl sulfoxide vehicle: Sp 5,6-DCl-cBIMPS (10 μM; BIOLOG Life Science); forskolin (100 μM; Sigma); tetradecanoylphorbol-acetate (100 nM; Sigma); okadaic acid (200 nM; Sigma); and tautomycetin (10 nM; Tocris). Mice were injected intraperitoneally with the following drugs or with their vehicle (9 g l−1 NaCl) caffeine (7.5 mg kg−1, Sigma) and cocaine (10 or 20 mg kg−1, Coper).
Engmann O., Giralt A., Gervasi N., Marion-Poll L., Gasmi L., Filhol O., Picciotto M.R., Gilligan D., Greengard P., Nairn A.C., Hervé D, & Girault J.A. (2015). DARPP-32 interaction with adducin may mediate rapid environmental effects on striatal neurons. Nature Communications, 6, 10099.
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2

Protein Phosphorylation Quantification

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Primary antibodies were: Mouse monoclonal for DARPP-32 5a and 658 (link) (1/5,000), calmodulin (Upstate Cell Signaling # 05-173); rabbit polyclonal for pThr34, pThr75, pSer97 (#12438, #2301, #3401; 1:2,000; Cell Signaling); total β-adducin (#AP20545PU-N, 1:500; Santa Cruz, Acris), pSer713 of β-adducin (also reacts with pSer662 of γ-adducin and pSer724 of α-adducin; #05-587; 1:1,000; Chemicon/Millipore); β-actin (#A5316; 1:1,000; Sigma); myc-tag (#05-724; 1:500; Millipore), GFP (Abcam #ab6556, 1/1,000), pThr79-pSer81-calmodulin (Abcam, #ab194526), pSer10 histone H3 (#06-570; rabbit; Millipore). Secondary antibodies comprised IRDye800-conjugated anti-mouse and anti-rabbit (#610-132-121, #611-132-122; both 1:4,000; Rockland) antibodies for immunoblotting and anti-mouse CY3-conjugated antibody (#A10521; 1:400; Molecular Probes) for immunochemistry. COS-7-cells were treated with one or the combination of the following compounds, as indicated, or with DMSO-vehicle: Sp 5,6-DCl-cBIMPS (10 μM; BIOLOG life science), forskolin (100 μM; Sigma), tetradecanoylphorbol-acetate (TPA, 100 nM; Sigma), okadaic acid (200 nM; Sigma), tautomycetin (10 nM; Tocris). Mice were injected intraperitoneally with the following drugs or with their vehicle (9 g l−1 NaCl) caffeine (7.5 mg kg−1, Sigma) and cocaine (10 or 20 mg kg−1, Coper).
Engmann O., Giralt A., Gervasi N., Marion-Poll L., Gasmi L., Filhol O., Picciotto M.R., Gilligan D., Greengard P., Nairn A.C., Hervé D, & Girault J.A. (2015). DARPP-32 interaction with adducin may mediate rapid environmental effects on striatal neurons. Nature Communications, 6, 10099.
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