Co3 protoporphyrin 9

Arachidonic acid (AA; 5Z, 8Z, 11Z, 14Z-eicosatetraenoic acid) and aspirin were purchased from Cayman Chemical Company (Ann Arbor, MI). Celebrex® was from a physician sample. [acetyl-¹⁴C] aspirin (55 mCi/mmol) was purchased from American Radiolabeled Chemicals (St. Louis, MO). Fe³⁺-protoporphyrin IX and Co³⁺-protoporphyrin IX were purchased from Frontier Scientific (Logan, UT). Decyl maltoside (C₁₀M) was purchased from Anatrace (Maumee, OH), while n-octyl-β-D-glucopyranoside (βOG) was purchased from Inalco Pharmaceuticals (San Luis Obispo, CA). Anti-FLAG M2 affinity resin was purchased from Sigma-Aldrich (St. Louis, MO). FLAG peptide was purchased from GenScript (Piscataway, NJ). Polyacrylic acid (sodium salt) 5100 was purchased from Hampton Research. Restriction enzymes were purchased form New England Biolabs. The QuikChange™ II mutagenesis kit was purchased from Agilent Technologies (Santa Clara, CA). Oligos for site-directed mutagenesis, the Bac-to-Bac® Baculovirus expression kit and associated reagents, including fungizone and penicillin-streptomycin, were purchased from Invitrogen (Carlsbad, CA). SFM 921 serum free media, Spodoptera frugiperda 21 (Sf21) insect cells, and fetal bovine serum were purchased from Expression Systems (Davis, CA). HiTrap HP chelating and HiPrep Sephacryl 300-HR chromatography columns were purchased from GE Healthcare.