Jm1e3
The JM1E3 is a laboratory instrument designed for cell sorting and analysis. It is capable of detecting and separating cells based on their physical and fluorescent characteristics. The core function of the JM1E3 is to provide researchers with a tool for analyzing and sorting cell populations in a research setting.
Lab products found in correlation
4 protocols using jm1e3
Activation of Porcine Aortic Endothelial Cells
Porcine Alveolar Epithelial Cell Activation
Evaluating SLA-I and SLA-II Silencing in ICCs
Silencing of Porcine SLA Class I and II
Total RNA was isolated from three SLA-silenced PTEC lines and reversed transcribed using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). qRT-PCR was utilized to detect β2m, CIITA, and SLA-DRα transcript level expression using Taqman assays (Ss03391156_m1; Ss06941905_g1; Ss03389945_m1; all Thermo Fisher Scientific), respectively. GAPDH (Ss03375629_u1, Thermo Fisher Scientific) expression was used as an endogenous control.
SLA class I and class II protein expression was determined by flow cytometry. Cell staining was performed using an anti-SLA class I antibody (JM1E3; Bio-Rad Laboratories GmbH, Hercules, CA, USA) and anti-SLA class II DQ antibody (K274.3G8; Bio-Rad Laboratories GmbH) followed by APC-conjugated anti-mouse IgG1 (RMG1-1; Invitrogen) or AlexaFluor488-conjugated goat anti-mouse IgG secondary antibody (Invitrogen) staining.
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