Pd l2 pe

Manufactured by Thermo Fisher Scientific

Sourced in Canada

The PD-L2–PE is a fluorescently labeled antibody reagent that binds to the PD-L2 (Programmed Death-Ligand 2) protein. It is designed for use in flow cytometry applications to detect and quantify the expression of PD-L2 on cell surfaces.

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Lab products found in correlation

Ghost dye violet 510, by Cytek Biosciences (1 mentions) Tcs sp5 microscope, by Leica (1 mentions) Fc blocker, by BioLegend (1 mentions) Igm apc, by Thermo Fisher Scientific (1 mentions) Igm af350, by Thermo Fisher Scientific (1 mentions) Live cell imaging solution, by Thermo Fisher Scientific (1 mentions) Cd11b, by Thermo Fisher Scientific (1 mentions) Lsr 2, by BD (1 mentions) Cd80 pe, by Thermo Fisher Scientific (1 mentions) Cd86 pe, by Thermo Fisher Scientific (1 mentions) Cd4 rm4 5 pe cy7, by BD (1 mentions) Mtor mab 7c10, by Cell Signaling Technology (1 mentions) Gfp d5.1 mab, by Cell Signaling Technology (1 mentions) Apc conjugated donkey anti rabbit igg, by Jackson ImmunoResearch (1 mentions) B220 fitc, by BD (1 mentions) Cxcr3 apc, by Thermo Fisher Scientific (1 mentions) Cd11b pe cy7, by BD (1 mentions) Cd11c apc, by BD (1 mentions) Cd19 pe cy7, by BD (1 mentions) Pd l1 pe, by BD (1 mentions)

Spelling variants of this product

PD-L2 (6 citations) PE anti-human PD-L2 (1 citations)

2 protocols using pd l2 pe

Multicolor Flow Cytometry Analysis

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Cells were incubated with Fc Blocker (BioLegend, CA) for 15 min and then stained with the following antibodies for 45 min: B220–PerCp–Cy5.5, CD5–PECy7, Cd11b–e780 or Cd11b APC–Cy7, CD19–e450, PD-L2–PE (eBioscience, CA), Ghost Dye Violet 510 (Tonbo Biosciences, CA), IgM–AF350 (Life Technologies, CA), or IgM–APC (eBioscience, CA). Cells were then washed three times by centrifugation and resuspended in Live Cell Imaging Solution (Life Technologies, CA). FACS data were collected with BD LSRII and analyzed using FlowJo software (TreeStar, OR). Fluorescent images were taken using a 63× oil-immersion objective with a Leica TCS SP5 microscope.

Lee R., Mao C., Vo H., Gao W, & Zhong X. (2015). Fluorescence tagging and inducible depletion of PD-L2–expressing B-1 B cells in vivo. Annals of the New York Academy of Sciences, 1362(1), 77-85.

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Comprehensive Immune Cell Profiling

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The following antibodies were used: mTOR mAb (7C10), phospho-S6 (Ser235/236) (D57.2.2E)-APC, phospho-p70 S6 kinase (Thr389) (108D2) mAb, phosphor-AKT (Thr308) (C31E5E) mAb, phospho-Akt (Ser473) (D9E) mAb, GFP (D5.1) mAb, SHP-2 (D50F2) mAb (Cell Signaling Technology); APC-conjugated Donkey-anti-Rabbit IgG (Jackson ImmunoResearch); CD3e (145-2C11)-FITC, CD4 (RM4-5)-PE-Cy7, Vβ4 (KT4)-PE, CD11c-APC, CD11b-PE-Cy7, B220-FITC, CD19-PE-Cy7 and PD-L1-PE (BD Biosciences); F4/80-PE and PD-1-PE-Cy7 (BioLegend); CD71-APC and CD98-PE, CD90.1 (Thy-1.1)-PE, CXCR3-APC, CD80-PE, CD86-PE and PD-L2-PE (eBioscience); PD-1 (J43) Ab (Novus); T-bet-FITC and SH-PTP2 (C-18) (Santa Cruz Biotechnology).

Chen W., Wan X., Ukah T.K., Miller M.M., Barik S., Cattin-Roy A.N, & Zaghouani H. (2016). Antigen-Specific Immune Modulation Targets mTORC1 Function to Drive Chemokine Receptor-Mediated T Cell Tolerance. Journal of immunology (Baltimore, Md. : 1950), 197(9), 3554-3565.

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