Z yvad fmk

Antibodies: anti-IL-1β (NB600-633, Novus Biologicals, Littleton, CO, USA); anti-HA (Ab137838, Abcam, Cambridge, UK); anti-Flag (F1804, Sigma-Aldrich, St Louis, MO, USA); anti-NLRP3 (AG-20B-0014-C100, Adipogen, San Diego, CA, USA); anti-ASC (AG-25B-0006-C100, Adipogen, San Diego, CA, USA)
Reagents: recombinant human IL-1β (IL038, Sigma-Aldrich, St Louis, MO, USA), recombinant human TNF-α (ab9642, Abcam, Cambridge, UK); Z-YVAD-FMK (S8507, Selleck, Shanghai, China); Z-VAD-FMK (S7023, Selleck, Shanghai, China); MCC950 (S8930, Selleck, Shanghai, China); Bay 11-7082 (S2913, Selleck, Shanghai, China); IL-1R antagonist (sc-221747, Santa Cruz Biotechnology, Santa Cruz, CA, USA).

HT22 cells and C8-B4 cells were obtained from the Kunming Institute of Zoology (Kunming, China). Antibodies against NLRP3, GSDMD-N, IL-18, and XBP-1 were purchased from Abcam (Cambridge, MA, USA). Antibodies against XBP-1s and Cleaved Caspase-1 were purchased from Cell Signaling Technology (Massachusetts, USA). Antibody against Cleaved IL-1β was purchased from Affinity Biosciences (Jiangsu, China). Antibody against beta-actin was purchased form Proteintech Group, Inc. (USA). Z-YVAD-FMK was purchased from Selleckchem (Selleck Chemicals, China). Polyphyllin VI was purchased from MCE (MedChemExpress, USA).