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1 pyrenebutyric acid n hydroxysuccinimide ester

Manufactured by Merck Group

1-pyrenebutyric acid N-hydroxysuccinimide ester is a chemical compound commonly used as a fluorescent label in biochemical applications. It serves as a reactive ester that can covalently attach to amine-containing molecules, enabling their detection and analysis through fluorescence techniques.

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2 protocols using 1 pyrenebutyric acid n hydroxysuccinimide ester

1

Synthesis of Fluorescent PEG Conjugates

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Fmoc-protected amino acids and coupling agents were purchased from Iris Biotech GmbH. α-Methoxy-ω-amino PEG (CH3O–PEG–NH2, Mn = 5000 g mol−1) was purchased from Rapp Polymere. 1,4,5,8-Naphthalenetetracarboxylic dianhydride, 1-pyrenebutyric acid N-hydroxysuccinimide ester, (1R,8S,9s)-bicyclo[6.1.0]non-4-yn-9-ylmethyl N-succinimidyl carbonate and other chemicals were purchased from Sigma-Aldrich. Solvents were purchased from several departmental suppliers, Honeywell, Fisher and Sigma-Aldrich.
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2

Enzyme-Catalyzed Functionalization of Carbon Nanotubes

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Sodium phosphate dibasic (Na 2 HPO 4 , ≥ 99%), sodium dihydrogen phosphate (NaH 2 PO 4 , ≥ 99%), hydrogen peroxide (≥ 30% w/w in H 2 O), 1-methyl-2-pyrrolidone (NMP, ≥ 99%), pyrene (98%, py), 1-pyrenebutyric acid N-hydroxysuccinimide ester (pyNHS, 95 %), 1pyrenemethylamine hydrochloride (pyNH 2, 95 %), 1-pyrenebutyric acid (pyBA, 97%), 1pyreneacetic acid (pyAA, 97 %), and horseradish peroxidase from Armoracia rusticana (type II, 150-250 units/mg solid) were purchased from Sigma Aldrich and used as received without further purification. The enzyme was stored at -20 °C when not in use. Distilled water was purified to a minimum resistivity of 15 MΩ cm -1 using a Millipore Ultrapure system.
Commercial grade thin multi-walled carbon nanotubes (MWCNTs, Ø = 9.5 nm, 90% purity, NC7000, batch 1166) were obtained from Nanocyl and used as received without purification.
High purity oxygen and argon were obtained from Messer. N, N-Dimethylformamide (DMF, 99.8%) was obtained from Carlo Erba reagents (France). Acetonitrile (ACN) was obtained from VWR chemicals (France). The 1 % bacterial cellulose nanofibers gel was obtained from Nano Novin Polymer Co. (99%, average 40 nm diameter) and used as received.
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