P acetm mdq

Manufactured by Beckman Coulter

Sourced in United States

The P/ACETM MDQ is a laboratory instrument designed for the analysis of amino acids, peptides, and proteins. It utilizes principles of capillary electrophoresis to separate and detect these biomolecules.

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Lab products found in correlation

32 karat software, by Beckman Coulter (1 mentions)

Spelling variants of this product

P/ACE MDQ (35 citations) P/ACE (7 citations) P/ACETM MDQ system (4 citations) P/ACETM MDQ electrophoresis system (2 citations)

4 protocols using p acetm mdq

Quantifying Myocardial Tetrahydrobiopterin

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The supernatant obtained in point 2.3.1. was diluted 1:10 with 0.01M NaOH before starting the analysis. The determination of BH4 levels in ventricular tissue was performed by capillary zone electrophoresis with UV-Vis detection by diode array, using the methodology of Han [28 (link)].
The sample was passed through a Sep-Pak Classic C18-NH2 cartridge (Waters, Urbana, IL, USA) and directly analyzed with a P/ACETM MDQ (Beckman Coulter Inc., Fullerton, CA, USA) at 30 kV for 6 min and a wavelength of 230 nm at 10 °C, using a running buffer (0.1 M Tris—0.1 M boric acid—2 mM EDTA, pH 8.75). The samples were injected under hydropneumatic pressure of 0.5 psi/10 s. BH4 concentration was determined using a standard curve from 0–200 pmol/mL using analytical grade BH4.

Castrejón-Téllez V., del Valle-Mondragón L., Pérez-Torres I., Guarner-Lans V., Pastelín-Hernández G., Ruiz-Ramírez A., Díaz-Juárez J.A., Varela-López E., Oidor-Chan V.H., Vargas-González A., Martínez-Memije R., Flores-Chávez P., León-Ruíz B., Arriaga-Carrillo S, & Torres-Narváez J.C. (2022). TRPV1 Contributes to Modulate the Nitric Oxide Pathway and Oxidative Stress in the Isolated and Perfused Rat Heart during Ischemia and Reperfusion. Molecules, 27(3), 1031.

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Capillary Electrophoresis for Analyte Separation

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All CE experiments were carried out using a capillary electrophoresis system with a diode array detector from Beckman Coulter Instruments, model P/ACETM MDQ (Fullerton, CA, USA), operating at 214 nm. The 32 Karat™ software, also from Beckman, was used to control the instrument and for data acquisition. Samples and standards were hydrodynamically injected (5 s at 0.5 psi) at the short end of a fused silica capillary with polyimide coating (50 cm total length, 10.2 cm effective length, 50 µm i.d.). Before the first use, the capillary was rinsed with aqueous 1.0 mol L⁻¹ NaOH for 20 min, followed by ultrapure water for 20 min. Every day before starting the analysis, the capillary was rinsed with running buffer for 5 min.
The separation was accomplished using BGE containing 5 mmol L⁻¹ imidazole, 6 mmol L⁻¹ HIBA, and 23.3 mmol L⁻¹ 18-crown-6 in 3.7 % (v/v) methanol. A constant voltage of +11.4 kV was applied on the injection side (short end) with temperature fixed at 20 °C.

Klassen A., Fernandes R.F., de Oliveira D.C., do Nascimento M.P., Borges M.M., de Oliveira M.A., Calixto L.A, & Borges K.B. (2022). Short-End Injection Capillary Electrophoresis and Multivariate Analysis for Simultaneous Determination of Heavy Metals in Passiflora incarnata Tea. International Journal of Environmental Research and Public Health, 19(23), 15994.

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Serum Tetrahydrobiopterin Measurement

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50 μL of serum were employed to measure the concentration of serum tetrahydrobiopterin (BH4) by capillary electrophoresis, with fluorescence emission created with laser excitation (Capillary Electrophoresis Systems P/ACETM MDQ, Beckman Coulter Inc., USA.)

Montes-Rivera J., Arellano-Mendoza M., Nájera N., Del Valle-Mondragón L., Villarreal F., Rubio-Gayosso I., Perez-Duran J., Meaney E, & Ceballos G. (2019). Effect of (-)-epicatechin on the modulation of progression markers of chronic renal damage in a 5/6 nephrectomy experimental model. Heliyon, 5(4), e01512.

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Simultaneous Determination of Angiotensin II and Angiotensin-(1-7) in Biological Samples

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Ang II and Ang–(1-7) were simultaneously determined in the rat serum or lung homogenate (50 μL) by capillary zone electrophoresis with UV detection by the photodiode array. Samples were deproteinized with cold methanol and 20% trichloroacetic acid in a 10:1 ratio, then were centrifuged at 16,000× g for 15 min at 10 °C and filtered with 0.22 µm nitrocellulose membrane filters, diluted 1:10 with cold 0.1 M sodium hydroxide. The samples were passed through Cold Sep-Pak Classic C-18 cartridges and tested directly on the P/ACE^TM MDQ de Beckman Coulter system for previous capillary precondition by passing 1.0 M sodium hydroxide solution for 30 min, then deionized water for 30 min, and finally the running buffer (100 mM boric acid + 3 mM tartaric acid + 10 fM gold III chloride at pH 9.8) for 30 min. Samples were injected under hydrodynamic pressure at 0.5 psi/10 s. Separation was performed at 30 kV for 10 min at 200 nm at 20 °C. The capillary was washed between runs with 1.0 M sodium hydroxide for 2 min, deionized water for 2 min, and running buffer for 4 min. The results were expressed in pmoles/mL. Ang II and Ang 1-7 concentrations were determined using a standard curve [62 (link)].

Sánchez-Gloria J.L., Martínez-Olivares C.E., Del Valle-Mondragón L., Cortés-Camacho F., Zambrano-Vásquez O.R., Hernández-Pando R., Sánchez-Muñoz F., Sánchez-Lozada L.G, & Osorio-Alonso H. (2023). Allicin, an Emerging Treatment for Pulmonary Arterial Hypertension: An Experimental Study. International Journal of Molecular Sciences, 24(16), 12959.

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