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Enzymatic colorimetric kit

Manufactured by DiaSys
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Sourced in Germany

The Enzymatic colorimetric kit is a laboratory equipment product designed for the quantitative determination of analytes through a colorimetric enzymatic reaction. The kit provides the necessary reagents and materials to perform this analysis.

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Enzymatic kits

2 protocols using enzymatic colorimetric kit

1

Quantification of Liver Lipid Levels

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Liver lipids were extracted from 100 mg of tissue using chloroform-methanol, according to the method described by Folch et al. [18 (link)]. The organic layer was dried using liquid nitrogen and solubilized in isopropanol/Triton X-100 (10%). The concentrations of total cholesterol and triglycerides were measured with an enzymatic colorimetric kit (DiaSys Diagnostic Systems, Germany).
Meneses M.E., Martínez-Carrera D., Torres N., Sánchez-Tapia M., Aguilar-López M., Morales P., Sobal M., Bernabé T., Escudero H., Granados-Portillo O, & Tovar A.R. (2016). Hypocholesterolemic Properties and Prebiotic Effects of Mexican Ganoderma lucidum in C57BL/6 Mice. PLoS ONE, 11(7), e0159631.
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2

Extracting Liver Lipids and Quantifying Cholesterol and Triglycerides

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Liver lipids were extracted from 100 mg of tissue using chloroform-methanol, according to the method described by Folch et al. 22 The organic layer was dried using liquid nitrogen and solubilized in isopropanol/Triton X-100 (10%). The concentrations of total cholesterol and triglycerides were measured with an enzymatic colorimetric kit (DiaSys Diagnostic Systems, Germany).
Hernandez-Velazquez I ., Sanchez-Tapia M ., Ordaz-Nava G ., Torres N ., Tovar AR , & Galvez A . (2020). Black bean protein concentrate ameliorates hepatic steatosis by decreasing lipogenesis and increasing fatty acid oxidation in rats fed a high fat-sucrose diet. Food & function, 11(12).
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