The workflow of all the analyses is illustrated in Fig. 1 . Individual target PACs, internal and recovery standards (IS and RS, respectively), their abbreviations, predicted log Kow, vendors, and purities are listed in the Supplementary Material (SM) (Table S1 ). Targeted chemical analysis included 87 PACs. Acetone (≥99.8%), n-hexane (≥98%), toluene (>99.7%), and anhydrous sodium sulfate (Na2SO4) (99%) were purchased from VWR (Stockholm, Sweden). Dichloromethane (99.8%) and formic acid (98–100%) were purchased from Sigma Aldrich (Stockholm, Sweden). Methanol (>99%, HPLC grade) and ultrapure water (LC-MS grade) were purchased from Fisher Scientific (Göteborg, Sweden). Calcium chloride and sodium azide were purchased from Merck (Darmstadt, Germany). Strips of polyoxymethylene (POM) (76 μm thickness) were purchased from CS Hyde (Lake Villa, IL, USA).
Dimethyl sulfoxide (DMSO) (99.9%), 17β-estradiol (E2) standard (≥98%), flutamide standard (≥99%) and dihydrotestosterone (DHT) (≥99%) were purchased from Sigma Aldrich (Stockholm, Sweden); 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) standard (99.1%) was purchased from AccuStandard (New Haven, CT, USA); and steadylite plus™ reporter gene assay system was purchased from PerkinElmer (Hägersten, Sweden).
Dimethyl sulfoxide (DMSO) (99.9%), 17β-estradiol (E2) standard (≥98%), flutamide standard (≥99%) and dihydrotestosterone (DHT) (≥99%) were purchased from Sigma Aldrich (Stockholm, Sweden); 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) standard (99.1%) was purchased from AccuStandard (New Haven, CT, USA); and steadylite plus™ reporter gene assay system was purchased from PerkinElmer (Hägersten, Sweden).