Inert cap wax

Manufactured by GL Sciences

Sourced in Japan

The Inert cap WAX is a laboratory equipment component designed to provide an inert and sealed environment for analytical applications. It serves as a protective cap to maintain the integrity of the sample or solution during storage and analysis.

Automatically generated - may contain errors

Lab products found in correlation

Qp2010, by Shimadzu (2 mentions) Cdd 10a, by Shimadzu (1 mentions) Ionpac cs12a, by Thermo Fisher Scientific (1 mentions) Ionpac cg12a, by Thermo Fisher Scientific (1 mentions) Shim pack scr 102h, by Shimadzu (1 mentions) 5975 msd mass spectrometer, by Agilent Technologies (1 mentions) Polydimethylsiloxane, by Merck Group (1 mentions) Db wax, by Agilent Technologies (1 mentions) G 5000, by Hitachi (1 mentions)

3 protocols using inert cap wax

Fecal Metabolite Quantification Protocol

Cited 1 time

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For this, 0.1 g of each fecal sample suspended in 2.5 mL of phosphate buffer including 0.4 mg/L of 4-isopropylphenol as an internal standard was employed to the previous procedures [12 (link)]. Briefly, 1 mL of the supernatant was dehydrated and purified with 3 cartridges such as sodium sulfate drying cartridge (Bond Elut LRC; Agilent Technologies, Tokyo, Japan), C18 cartridge (Smart SPE C18-30; AiSTI Science, Wakayama, Japan), and PSA cartridge (Smart SPE PSA-30; AiSTI Science).
The levels of indole and phenol were determined by a single quadrupole gas chromatograph–mass spectrometer (QP-2010; Shimadzu, Kyoto, Japan) equipped with a capillary column (Inert cap WAX; GL Science, Tokyo, Japan). Helium was used as the carrier gas. The injector and interface temperatures were maintained at 240 °C and 230 °C, respectively. For the analysis, 1 µL of the extract was subjected to the splitless mode. The mass spectrometer was operated in the electron impact ionization mode at 70 eV. The measurements were recorded, and data were obtained from the selected ion-monitoring mode for quantification.

Takeuchi H., Yoshikane Y., Takenaka H., Kimura A., Islam J.M., Matsuda R., Okamoto A., Hashimoto Y., Yano R., Yamaguchi K., Sato S, & Ishizuka S. (2022). Health Effects of Drinking Water Produced from Deep Sea Water: A Randomized Double-Blind Controlled Trial. Nutrients, 14(3), 581.

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Comprehensive Fecal Metabolite Analysis

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We measured faecal levels of organic acids, SCFAs, ammonium ion, indoles, phenol, skatole, and p-cresol to determine metabolite levels. We measured organic acids and SCFAs such as acetic acid, propionic acid, butyric acid, iso-butyric acid, succinic acid, lactic acid, formic acid, valeric acid, and iso-valeric acid using high-performance liquid chromatography (Prominence, Shimadzu, Kyoto, Japan) with a detector (CDD-10A, Shimadzu, Kyoto, Japan), two tandemly-arranged columns (Shim-pack SCR-102(H), 300 mm × 8 mm ID, Shimadzu, Kyoto, Japan), and a guard column (Shim-pack SCR-102(H), 50 mm × 6 mm ID, Shimadzu, Kyoto, Japan). We measured ammonium ion concentration using an ion chromatography system (ICS-1000, DIONEX) with a column (IonPac CS12A, 4 mm × 250 mm, DIONEX) and a guard column (IonPac CG12A, 4 mm × 50 mm, DIONEX). We measured indoles, phenol, skatole, and p-cresol using gas chromatography/mass spectrometry (QP-2010, Shimadzu, Kyoto, Japan) and a capillary column (Inert cap WAX, 30 m × 0.25 mm × 0.25 µm, GL science, Japan). Detailed information regarding the analysis of metabolites in faeces is provided in the supplementary file.

Saji N., Murotani K., Hisada T., Kunihiro T., Tsuduki T., Sugimoto T., Kimura A., Niida S., Toba K, & Sakurai T. (2020). Relationship between dementia and gut microbiome-associated metabolites: a cross-sectional study in Japan. Scientific Reports, 10, 8088.

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GC and GC-MS Analysis of Compounds

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GC analyses were performed on a G-5000 (Hitachi) equipped with a flame ionization detector with the following conditions: a fused silica capillary column (Inert Cap-Wax, 60 m × 0.25 mm, film thickness 0.25 µm, GL Sciences) and a chiral column (CP-Chirasil-Dex CB, 25 m × 0.25 mm, film thickness 0.25 µm); column temperature program: 60 ℃ for 2 min, increasing to 220 ℃ at a rate of 4 ℃/min, then held at 220℃ for 15 min; the injector was set at 60 ℃ and the detector was set at 230 ℃ [7] ; carrier gas: helium (1 ml/min); injection volume: 1 µl; split ratio: 99 : 1.
GC-MS and SPME were performed on an Agilent 6850 series gas chromatograph connected to an Agilent MSD 5975 mass spectrometer under the following operating conditions: fused silica capillary column (DB-WAX, 60 m × 0.25 mm, film thickness 0.25 µm, Agilent Technology); SPME fiber: 100 µm polydimethylsiloxane (Supelco); the column temperature program was the same as that used for the GC analysis; ionization energy: 70 eV; carrier gas: helium (1 mL/min); injection volume: 1 µl; split ratio: 99:1. The identities of most of the separated compounds were confirmed by comparison of the retention indices (RI) and mass spectra patterns (MS library: NIST 2 and flavors). RI was accordant with past data.

Hirai M, & Ito M. (2019). Sedative effects of the essential oil and headspace air of Ocimum basilicum by inhalation in mice. Journal of natural medicines, 73(1).

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