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Ritonavir

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Ritonavir is a laboratory reagent used in research applications. It functions as a protease inhibitor, which is a class of drugs that block the activity of certain enzymes involved in viral replication. Ritonavir is commonly used in research studies to investigate its potential effects on various biological processes.

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Lab products found in correlation

Emtricitabine, by Selleck Chemicals (2 mentions) Z vad fkm, by R&D Systems (2 mentions) Dolutegravir, by Selleck Chemicals (2 mentions) Anti pe magnetic beads, by Miltenyi Biotec (2 mentions) Interleukin 2 (il 2), by Thermo Fisher Scientific (2 mentions) Phytohemagglutinin (pha), by Merck Group (2 mentions) Tenofovir, by Selleck Chemicals (2 mentions) 8499 if 010 cf, by R&D Systems (1 mentions)

3 protocols using ritonavir

1

Solubilization of Remdesivir, Lopinavir, and Interferon

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Remdesivir (RDV), lopinavir (LPV), and ritonavir (RTV) were solubilized in 100% DMSO and provided by Gilead Sciences, Inc. Recombinant human interferon beta (IFNb) protein was purchased from R&D Systems (8499IF010/CF, 2.8 × 108 IU/mg compared with WHO standard) and solubilized in sterile water as recommended.
Sheahan T.P., Sims A.C., Leist S.R., Schäfer A., Won J., Brown A.J., Montgomery S.A., Hogg A., Babusis D., Clarke M.O., Spahn J.E., Bauer L., Sellers S., Porter D., Feng J.Y., Cihlar T., Jordan R., Denison M.R, & Baric R.S. (2020). Comparative therapeutic efficacy of remdesivir and combination lopinavir, ritonavir, and interferon beta against MERS-CoV. Nature Communications, 11, 222.
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2

Isolation and Analysis of HIV-Specific CD4+ T Cells

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CD4+ T cells were isolated from
~1×109 PBMCs by negative selection using the
Miltenyi CD4+ T isolation kit. Cells were cultured at
2×106/mL in R10 (RPMI supplemented with 10% heat
inactivated FCS, 10mM HEPES, 100U/mL PenStrep), and 25% volume
conditioned media. Conditioned media was made by culturing healthy PBMCs in R10
with PHA and IL-2 for 2 days, followed by a wash and 5 days in culture with IL-2
alone. The conditioned media was then collected and frozen at −80C until
use. 100U/mL IL-2 (Peprotech), 1ug/mL PHA (Sigma), 10uM Z-VAD-FKM (R&D),
10uM Ritonavir, 10uM Dolutegravir, 10uM Emtricitabine, 5uM Tenofovir, and 10uM
Maraviroc (all Selleckchem) were added to the media. 36h later, cells were
labeled with 5ug/mL each of biotinylated 3BNC117, 10-1074, PG16, followed by
Streptavidin PE (1:500, BD) and anti-PE magnetic beads (Miltenyi Biotech). Cells
were then passed over a magnetic column and bound cells were eluted for
downstream analysis. For FACS sorting, cells were labeled with the following
antibodies, all Biolegend: CD1c (cat. no. 331510), CD3 (cat. no. 300430), CD4
(cat. no. 317444), CD8 (cat. no. 344726), CD14 (cat. no. 301812), CD20 (cat. no.
302318), CD32a (cat. no. 303204), and CD56 (cat. no. 318314).
Cohn L.B., da Silva I.T., Valieris R., Huang A.S., Lorenzi J.C., Cohen Y.Z., Pai J.A., Butler A.L., Caskey M., Jankovic M, & Nussenzweig M.C. (2018). Clonal CD4+ T cells in the HIV-1 latent reservoir display a distinct gene profile upon reactivation. Nature medicine, 24(5), 604-609.
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3

Isolation and Analysis of HIV-Specific CD4+ T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
CD4+ T cells were isolated from
~1×109 PBMCs by negative selection using the
Miltenyi CD4+ T isolation kit. Cells were cultured at
2×106/mL in R10 (RPMI supplemented with 10% heat
inactivated FCS, 10mM HEPES, 100U/mL PenStrep), and 25% volume
conditioned media. Conditioned media was made by culturing healthy PBMCs in R10
with PHA and IL-2 for 2 days, followed by a wash and 5 days in culture with IL-2
alone. The conditioned media was then collected and frozen at −80C until
use. 100U/mL IL-2 (Peprotech), 1ug/mL PHA (Sigma), 10uM Z-VAD-FKM (R&D),
10uM Ritonavir, 10uM Dolutegravir, 10uM Emtricitabine, 5uM Tenofovir, and 10uM
Maraviroc (all Selleckchem) were added to the media. 36h later, cells were
labeled with 5ug/mL each of biotinylated 3BNC117, 10-1074, PG16, followed by
Streptavidin PE (1:500, BD) and anti-PE magnetic beads (Miltenyi Biotech). Cells
were then passed over a magnetic column and bound cells were eluted for
downstream analysis. For FACS sorting, cells were labeled with the following
antibodies, all Biolegend: CD1c (cat. no. 331510), CD3 (cat. no. 300430), CD4
(cat. no. 317444), CD8 (cat. no. 344726), CD14 (cat. no. 301812), CD20 (cat. no.
302318), CD32a (cat. no. 303204), and CD56 (cat. no. 318314).
Cohn L.B., da Silva I.T., Valieris R., Huang A.S., Lorenzi J.C., Cohen Y.Z., Pai J.A., Butler A.L., Caskey M., Jankovic M, & Nussenzweig M.C. (2018). Clonal CD4+ T cells in the HIV-1 latent reservoir display a distinct gene profile upon reactivation. Nature medicine, 24(5), 604-609.
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