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Odyssey clx ir scanner

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The Odyssey CLx IR scanner is a high-performance infrared imaging system designed for a wide range of applications in life science research. The Odyssey CLx utilizes advanced infrared detection technology to provide sensitive and quantitative analysis of protein and nucleic acid samples. The system features a compact design, flexible scan area, and intuitive software for efficient data acquisition and analysis.

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Lab products found in correlation

Trypsin, by Thermo Fisher Scientific (2 mentions) Alpha tubulin, by Santa Cruz Biotechnology (2 mentions) Ferritin light chain, by Santa Cruz Biotechnology (2 mentions) Gapdh, by Santa Cruz Biotechnology (2 mentions) Prism 7, by GraphPad (2 mentions)

2 protocols using odyssey clx ir scanner

1

Ferroptosis Inhibitor Screening in HT-1080 Cells

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0.8 million HT-1080 cells were seeded per well in a 60 mm plate and allowed to adhere overnight. Cells were then co-treated with 100 μM α-Tocopherol and either 1 μM of RSL3, 10 μM of Erastin, 5 μM of FIN56, 10 μM of FINO2, or vehicle for 10 h. Cells were harvested with trypsin (Invitrogen, #25200-114), pelleted, and frozen at -80˚C. Cell pellets were thawed, lysed, blotted, and imaged as previously described.3 (link) In particular, for this experiment, the set of 15 pellets was run on a single gel. Ultimately, two gels were run and quantified from a total of six biological replicates (30 pellets in total). For the other proteins of interest, experiments were performed in biological triplicate. Antibodies used were: GPX4 (abcam, #ab125066, 1:250 dilution), Ferritin Light Chain (Santa Cruz, #sc-390558, 1:1000 dilution), IRP2 (Nous Biological, #NB100-1798, 1:1000 dilution), Transferrin receptor 1 (CD71, TFRC, Cell Signaling, #13113, 1:1000 dilution), Actin (Cell signaling, #D18C11, 1:3000 dilution), alpha-tubulin (Santa Cruz, #sc-32293, 1:3000 dilution) and GAPDH (Santa Cruz, # sc-47724, 1:10,000 dilution). Results were quantified using a LI-COR Odyssey CLx IR scanner and GraphPad Prism 7.
Gaschler M.M., Andia A.A., Liu H., Csuka J.M., Hurlocker B., Vaiana C.A., Heindel D.W., Zuckerman D.S., Bos P.H., Reznik E., Ye L., Tyurina Y.Y., Lin A.J., Shchepinov M.S., Chan A.Y., Peguero-Pereira E., Fomich M.A., Daniels J.D., Bekish A.V., Shmanai V.V., Kagan V.E., Mahal L.K., Woerpel K.A, & Stockwell B.R. (2018). FINO2 Initiates Ferroptosis Through GPX4 Inactivation and Iron Oxidation. Nature chemical biology, 14(5), 507-515.
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2

Ferroptosis Inhibitor Screening in HT-1080 Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
0.8 million HT-1080 cells were seeded per well in a 60 mm plate and allowed to adhere overnight. Cells were then co-treated with 100 μM α-Tocopherol and either 1 μM of RSL3, 10 μM of Erastin, 5 μM of FIN56, 10 μM of FINO2, or vehicle for 10 h. Cells were harvested with trypsin (Invitrogen, #25200-114), pelleted, and frozen at -80˚C. Cell pellets were thawed, lysed, blotted, and imaged as previously described.3 (link) In particular, for this experiment, the set of 15 pellets was run on a single gel. Ultimately, two gels were run and quantified from a total of six biological replicates (30 pellets in total). For the other proteins of interest, experiments were performed in biological triplicate. Antibodies used were: GPX4 (abcam, #ab125066, 1:250 dilution), Ferritin Light Chain (Santa Cruz, #sc-390558, 1:1000 dilution), IRP2 (Nous Biological, #NB100-1798, 1:1000 dilution), Transferrin receptor 1 (CD71, TFRC, Cell Signaling, #13113, 1:1000 dilution), Actin (Cell signaling, #D18C11, 1:3000 dilution), alpha-tubulin (Santa Cruz, #sc-32293, 1:3000 dilution) and GAPDH (Santa Cruz, # sc-47724, 1:10,000 dilution). Results were quantified using a LI-COR Odyssey CLx IR scanner and GraphPad Prism 7.
Gaschler M.M., Andia A.A., Liu H., Csuka J.M., Hurlocker B., Vaiana C.A., Heindel D.W., Zuckerman D.S., Bos P.H., Reznik E., Ye L., Tyurina Y.Y., Lin A.J., Shchepinov M.S., Chan A.Y., Peguero-Pereira E., Fomich M.A., Daniels J.D., Bekish A.V., Shmanai V.V., Kagan V.E., Mahal L.K., Woerpel K.A, & Stockwell B.R. (2018). FINO2 Initiates Ferroptosis Through GPX4 Inactivation and Iron Oxidation. Nature chemical biology, 14(5), 507-515.
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