Mda d5

Chemicals and reagents COC, COC-d 3 , cocaethylene (CE), CE-d 3 , BE, BE-d 3 , MDEA-d 5 , and HMMA were obtained by LGC standards, ecgonine methyl ester (EME) and EME-d 3 were obtained from Cerilliant (Round Rock, TX, USA). HMMA-d 3 , MDMA, MDMA-d 5 , 3,4methylenedioxymethamphetamine (MDA), and MDA-d 5 were obtained from Sigma-Aldrich (St Louis, MO, USA). HMMA sulfate (HMMA-S) was produced as described previously. [17] Hydroxymethoxyethylamphetamine (HMEA) was produced as described previously. [18] MDEA was obtained from seized materials. Dilutions and storage of COC and its metabolites were done in acetonitrile, for MDMA and metabolites in methanol. Isolute C18 (500 mg, 3 mL) and HCX cartridges (130 mg, 3 mL) were obtained from Biotage (Uppsala, Sweden). Mixture (100 All PU samples were spiked on site with HMMA-d 3 to a final concentration of 100 ng/mL and stored in polystyrene containers and immediately put on ice until freezing (max 12 h).
US was collected from areas (fences, bushes, etc.) where pools of urine were visible on the surface. The characteristics (liquid content, colour, type of soil) differed from urine with small amounts of sand to humus with high organic content.