Phosphorylated phospho thr172 ampk

Manufactured by Cell Signaling Technology

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Phosphorylated (phospho)-Thr172 AMPK is a laboratory reagent used to detect the phosphorylation of the AMPK (AMP-activated protein kinase) protein at the threonine 172 residue. AMPK is a key sensor of cellular energy status and plays a crucial role in the regulation of metabolism.

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Lab products found in correlation

Ne per nuclear and cytoplasmic extraction kit, by Thermo Fisher Scientific (1 mentions) Total ampk, by Cell Signaling Technology (1 mentions) Sirt1, by Cell Signaling Technology (1 mentions) Pparα, by Abcam (1 mentions) Pro prep protein extraction solution, by iNtRON Biotechnology (1 mentions) β actin, by Merck Group (1 mentions) Keap1, by Santa Cruz Biotechnology (1 mentions) Cytochrome c oxidase 1, by Santa Cruz Biotechnology (1 mentions) Lamin b1, by Cell Signaling Technology (1 mentions) Pgc 1α, by Novus Biologicals (1 mentions) Peroxidase conjugated secondary antibody, by ZSGB-BIO (1 mentions) 3 3 diaminobenzidine tetrahydrochloride, by ZSGB-BIO (1 mentions) F4 80, by Bio-Rad (1 mentions) Ppara, by Abcam (1 mentions) Cpt1b, by Abcam (1 mentions)

Spelling variants of this product

Phospho-AMPK (108 citations) Phospho-AMPK (Thr172) (53 citations) Phosphorylated AMPK (19 citations)

2 protocols using phosphorylated phospho thr172 ampk

Quantitative Protein Analysis of Kidney Tissues

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Total proteins from whole kidney tissues and HK2 cells were extracted using a Pro-Prep Protein Extraction Solution (Intron Biotechnology, Gyeonggi-Do, Republic of Korea) according to the manufacturer’s instructions. For Nrf2 expression, nuclear proteins were prepared using the NE-PER nuclear and cytoplasmic extraction kit (Thermo Fisher Scientific, Rockford, IL, USA). Western blot analysis was performed using the following antibodies: Nrf2 (Santa Cruz Biotechnology Inc., Dallas, TX, USA), Keap1 (Santa Cruz Biotechnology Inc.), Lamin B1 (Cell Signaling Technology Inc., Danvers, MA, USA), HO-1 (Cell Signaling Technology Inc.), NQO-1 (Santa Cruz Biotechnology Inc.), SIRT1 (Cell Signaling Technology Inc.), total AMPK (Cell Signaling Technology Inc.), phosphorylated (phospho)-Thr¹⁷² AMPK (Cell Signaling Technology Inc.), PPARα (Abcam), PGC-1α (Novus Biologicals, Littleton, CO, USA), estrogen-related receptor α (ERRα) (Millipore), SOD1 (Enzo Life Sciences, Farmingdale, NY, USA), SOD2 (Abcam), cytochrome c oxidase I (Santa Cruz Biotechnology) and IV (Cell Signaling Technology Inc.), B-cell leukaemia/lymphoma 2 (BCL-2) (Santa Cruz Biotechnology); BCL-2-associaated X protein (BAX) (Santa Cruz Biotechnology) and β-actin (1:10000, Sigma).

Kim E.N., Lim J.H., Kim M.Y., Ban T.H., Jang I.A., Yoon H.E., Park C.W., Chang Y.S, & Choi B.S. (2018). Resveratrol, an Nrf2 activator, ameliorates aging-related progressive renal injury. Aging (Albany NY), 10(1), 83-99.

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Immunohistochemical Assessment of Adipose and Muscle Markers

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Paraffin sections (4 mm) were immunostained with antibodies against adipose differentiation-related protein (ADRP) (Abcam), F4/80 (Serotec), a-smooth muscle actin (Abcam), PPARa (Abcam), CPT1b (Abcam), or phosphorylated (phospho-) Thr 172 AMPK (Cell Signaling Technology). After incubating with primary antibody overnight at 4 C, the membranes were incubated with peroxidase-conjugated secondary antibodies (ZSGB-BIO, Beijing, China), and the signal was developed using 3,3'-diaminobenzidine tetrahydrochloride (ZSGB-BIO). Sections were then examined under a light microscope and digitized with a high-resolution camera.

Wu L., Liu C., Chang D.Y., Zhan R., Zhao M., Man Lam S., Shui G., Zhao M.H., Zheng L, & Chen M. (2021). The Attenuation of Diabetic Nephropathy by Annexin A1 via Regulation of Lipid Metabolism Through the AMPK/PPARα/CPT1b Pathway. Diabetes, 70(10).

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