Aldh1b1 shrna in prfp c rs plasmid vector

Manufactured by OriGene

Sourced in United States

ALDH1B1 shRNA in pRFP-C-RS plasmid vector is a laboratory tool used for gene silencing experiments. The vector contains a short hairpin RNA (shRNA) sequence targeting the ALDH1B1 gene and is designed for stable gene knockdown in mammalian cell lines.

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Lab products found in correlation

Fugene hd transfection reagent, by Promega (1 mentions)

Close spelling variants of this product

PRFP-C-RS vector PRFP-C-RS ShRNA plasmid ShRNA vector

2 protocols using aldh1b1 shrna in prfp c rs plasmid vector

ALDH1B1 Knockdown in Panc1 Cells

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ALDH1B1 shRNA in pRFP-C-RS plasmid vector and pRS vector-negative control (scramble) were purchased from OriGene (Rockville, MD, USA, # TF314844). Stable clones were generated by transfecting Panc1 cells in a 6-well plate with 1 μg of each of the shRNA plasmids using Clontech transfection reagent (Clontech, Mountain View, CA, USA) according to the manufacturer’s recommendations. Successful knockdown of ALDH1B1 was confirmed by Western blot analysis.

Singh S., Arcaroli J.J., Orlicky D.J., Chen Y., Messersmith W.A., Bagby S., Purkey A., Quackenbush K.S., Thompson D.C, & Vasiliou V. (2016). ALDH1B1 as a Modulator of Pancreatic Adenocarcinoma. Pancreas, 45(1), 117-122.

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Stable Knockdown of ALDH1B1 in SW480 Cells

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ALDH1B1 shRNA in pRFP-C-RS plasmid vector and pRS vector-negative control (scramble) were purchased from OriGene (Rockville, MD). Stable clones were generated by transfecting SW480 cells in a 6-well plate with 1μg of each of the shRNA plasmids using FuGENE HD transfection reagent (Promega) according to the manufacturer’s recommendations.

Singh S., Arcaroli J., Chen Y., Thompson D.C., Messersmith W., Jimeno A, & Vasiliou V. (2015). ALDH1B1 Is Crucial for Colon Tumorigenesis by Modulating Wnt/β-Catenin, Notch and PI3K/Akt Signaling Pathways. PLoS ONE, 10(5), e0121648.

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