Log In Sign up
The largest database of trusted experimental protocols

Af647 goat anti mouse igg

Manufactured by Thermo Fisher Scientific
Visit Supplier
Sourced in Japan, United States

AF647 goat anti-mouse IgG is a fluorescently labeled secondary antibody used to detect the presence of mouse immunoglobulin G (IgG) in various experimental procedures. The antibody is conjugated with the Alexa Fluor 647 dye, which emits fluorescence in the far-red region of the visible spectrum.

Automatically generated - may contain errors

Lab products found in correlation

Axio scan z1, by Zeiss (2 mentions) Goat anti rabbit igg af488, by Thermo Fisher Scientific (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (2 mentions) Goat anti rabbit igg af555, by Thermo Fisher Scientific (2 mentions) Apc anti mouse cd45, by BioLegend (2 mentions) Af647 goat anti rat igg, by Thermo Fisher Scientific (2 mentions) Af488 donkey anti rabbit igg, by Thermo Fisher Scientific (2 mentions) Microscope imaging system, by Olympus (1 mentions) Imaris 8, by Oxford Instruments (1 mentions) Donkey anti goat igg af647, by Thermo Fisher Scientific (1 mentions) Poly l lysine coated glass coverslips, by BD (1 mentions) Axiovision 4, by Zeiss (1 mentions) Rabbit anti pad4, by Abcam (1 mentions) Rabbit anti mpo, by Agilent Technologies (1 mentions) Axioplan 2 imaging fluorescence microscope, by Zeiss (1 mentions) Af488 goat anti mouse igm, by Thermo Fisher Scientific (1 mentions) Clsm 710, by Zeiss (1 mentions)

Close spelling variants of this product

F(ab')2-Goat anti-Mouse IgG (H+L)AF647 Goat anti-mouse AF647 Anti-mouse IgG AF647 Anti-mouse AF647 Mouse anti-IgG

7 protocols using af647 goat anti mouse igg

1

Immunofluorescent Imaging of Human GBM, Normal Brain, and Mouse Brain

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sections were cut from Optimal Cutting Temperature (OCT) compound-embedded human GBM, human adjacent normal brain, and mouse brain tissues (5–6 µm thickness).
Tissue sections were stained using 5 µg/mL purified mouse anti-human CD3 (UCHT1; BioLegend) and 4 µg/mL purified hamster anti-mouse CD31 (2H8; Thermo Fisher Scientific) with goat anti-mouse IgG AF647 (Thermo Fisher Scientific) and goat anti-hamster IgG AF488 (Abcam) detection antibodies, and mouse anti-GD2-FITC (14.G2A; BD) at a dilution of 1:100. GFAP was detected with polyclonal rabbit-anti human GFAP (1:500, Agilent) and donkey anti-rabbit IgG AF55 detection (1:500, Thermo Fisher Scientific).
Whole-slide imaging was performed on a Zeiss Axio Scan.Z1 slide-scanner using 20× objective and ZEN 3.1 Blue system software. Fluorescence overlays were created by merging channels and applying false color using FIJI (ImageJ, National Institutes of Health).
For further detail see online supplemental methods.
Gargett T., Ebert L.M., Truong N.T., Kollis P.M., Sedivakova K., Yu W., Yeo E.C., Wittwer N.L., Gliddon B.L., Tea M.N., Ormsby R., Poonnoose S., Nowicki J., Vittorio O., Ziegler D.S., Pitson S.M, & Brown M.P. (2022). GD2-targeting CAR-T cells enhanced by transgenic IL-15 expression are an effective and clinically feasible therapy for glioblastoma. Journal for Immunotherapy of Cancer, 10(9), e005187.
+ Open protocol
+ Expand
2

Immunofluorescence Assay of Cell Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
For the immunofluorescence assay, cells were fixed, permeabilized at 4 °C for 30 min, incubated with the indicated antibodies (rabbit anti-ACE2 pAb, Proteintech, 21115-1-AP, 1:50; mouse anti-ASGPR1 mAb, Sino Biological, 10773-MM02, 1:50; rabbit anti-ASGPR1 mAb, Sino Biological, 10773-R011, 1:50; and mouse anti-KREMEN1 mAb, homemade, 1:100) at 4 °C overnight. The cells were washed twice with PBS, stained with labeled secondary antibodies (goat anti rabbit IgG/AF488, Thermo Fisher, A11034; goat anti mouse IgG/AF647, Thermo Fisher, A21236; goat anti human IgG/AF647, Thermo Fisher, A21455; goat anti mouse IgG/AF594, Thermo Fisher, A11005; each at 1:200) at 4 °C for 2 h, and subjected to 4′6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich) staining for observation under a microscope imaging system (Olympus, Japan).
Gu Y., Cao J., Zhang X., Gao H., Wang Y., Wang J., He J., Jiang X., Zhang J., Shen G., Yang J., Zheng X., Hu G., Zhu Y., Du S., Zhu Y., Zhang R., Xu J., Lan F., Qu D., Xu G., Zhao Y., Gao D., Xie Y., Luo M, & Lu Z. (2021). Receptome profiling identifies KREMEN1 and ASGR1 as alternative functional receptors of SARS-CoV-2. Cell Research, 32(1), 24-37.
+ Open protocol
+ Expand
3

Transparent Optic Nerve Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols
iDISCO was carried out as previously described (http://www.idisco.info)22 (link). The following antibodies were used: goat anti-mouse VEGFR3 (R&D, No. AF743,1:400), rat anti-mouse LYVE1 (R&D, MAB2125,1:400), rabbit anti-mouse LYVE1 (AngioBio, No. 11-034,1:200), mouse anti-human VEGFR3 (Santa Cruz Biotechnology, SC-28297, 1:200), rabbit anti-human LYVE1 (Angio-Proteomie, 102-PA50S, 1:200), goat anti-mouse IgG–AF647 (Invitrogen, A21235, 1:500), donkey anti-goat IgG–AF647 (Invitrogen, A21447, 1:500), goat anti-rabbit IgG–AF555 (Invitrogen, A21428, 1:500). Subsequently, the transparent optic nerves with optic nerve sheaths were imaged using a Leica confocal microscope (Stellaris 8). Three-dimensional rendering was completed using Imaris 8 software (Oxford Instruments).
Yin X., Zhang S., Lee J.H., Dong H., Mourgkos G., Terwilliger G., Kraus A., Geraldo L.H., Poulet M., Fischer S., Zhou T., Mohammed F.S., Zhou J., Wang Y., Malloy S., Rohner N., Sharma L., Salinas I., Eichmann A., Thomas J.L., Saltzman W.M., Huttner A., Zeiss C., Ring A., Iwasaki A, & Song E. (2024). Compartmentalized ocular lymphatic system mediates eye–brain immunity. Nature, 628(8006), 204-211.
+ Open protocol
+ Expand
4

Visualizing Neutrophil Extracellular Traps

Check if the same lab product or an alternative is used in the 5 most similar protocols
The 5 × 104 isolated PMNs were seeded on poly-L-lysine-coated glass coverslips (BD Biosciences, San Jose, CA, USA) in tissue-culture wells and allowed to settle before stimulation, as described earlier. Coverslips were rinsed with ice-cold HBSS and the cells fixed with 4% paraformaldehyde and blocked overnight (HBSS with 10% goat serum, 1% BSA, 0.1% Tween20, and 2 mM EDTA) at 4°C. NETs were detected with rabbit anti-NE (Abcam, Cambridge, MA, USA), rabbit anti-MPO (Dako, Glostrup, Denmark), two different rabbit anti-PAD 4 (Abcam), mouse anti-PAD4 (Abcam), mouse anti-histone H1 + core proteins (EMD Millipore, Billerica, MA, USA), and rabbit anti-citrullinated histone H3 (citH3, Abcam). Secondary antibodies were goat anti-rabbit IgG AF555, goat anti-rabbit IgG AF488 (Invitrogen Life Technologies, San Diego, CA, USA), and goat anti-mouse IgG AF647. DNA was stained with 4′,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich), and NETs were visualized by using a Zeiss Axioplan 2 Imaging fluorescence microscope in conjunction with a Zeiss AxioCam MRm monochromatic CCD camera and analyzed with Axiovision 4.8.2 software (Carl Zeiss). A minimum of 20 fields (at least 1,000 PMNs) per case was evaluated for MPO/NE and DNA co-staining; nuclear phenotypes and NETs were counted and expressed as percentage of the total number of cells in the fields.
Sur Chowdhury C., Giaglis S., Walker U.A., Buser A., Hahn S, & Hasler P. (2014). Enhanced neutrophil extracellular trap generation in rheumatoid arthritis: analysis of underlying signal transduction pathways and potential diagnostic utility. Arthritis Research & Therapy, 16(3), R122.
+ Open protocol
+ Expand
5

Immunofluorescence Staining of Cardiomyocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cardiomyocytes were fixed with pre-chilled methanol for 10 minutes, then washed twice using PBST (0.1% tween-20 in 1×PBS) with 5 minutes intervals. Cells were blocked for 1 hour at room temperature with blocking buffer (5% BSA in PBST) and incubated with primary antibodies overnight at 4°C. The primary antibodies were: Detyrosinated α-tubulin (Abcam, ab48389, used at 1:200), α-tubulin (CST, 3873S, used at 1:200), MARK4 (Abcam, ab124267, used at 1:200), APC anti-mouse CD45 (BioLegend, 103112, used at 1:200) and rabbit IgG isotype control (Novus Biologicals, NB810-56910, used at 1:2000). The cells were then washed with PBST and incubated with secondary antibody for 1 hour at room temperature. The secondary antibodies were: AF488 donkey anti-rabbit IgG (Invitrogen, A21206, used at 1:200), AF647 goat anti-mouse IgG (Invitrogen, A21236, used at 1:200), AF647 goat anti-rat IgG (Invitrogen, A21247, used at 1:200). DAPI (Sigma, 10236276001, used at 1:1000) was used. Confocal images were obtained by Leica SP5 Confocal Laser Scanning Microscope, collected by LAS AF software (2.7.3.9723), and analyzed by ImageJ (v2.0) analyze tools.
Yu X., Chen X., Amrute-Nayak M., Allgeyer E., Zhao A., Chenoweth H., Clement M., Harrison J., Doreth C., Sirinakis G., Krieg T., Zhou H., Huang H., Tokuraku K., St Johnston D., Mallat Z, & Li X. (2021). MARK4 controls ischaemic heart failure through microtubule detyrosination. Nature, 594(7864), 560-565.
+ Open protocol
+ Expand
6

Quantification of Heparan Sulfate and C3c in Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were washed and fixed with paraformaldehyde 4%, then labeled with anti-HS (10E4, Amsbio, 370255, 5 μg/ml; 16 hours, 4 °C) and anti-C3c-fluorescein isothiocyanate (Dako, F020102-2, 12 μg/ml; 1 hour, RT). Anti-HS was revealed by AF488 goat anti-mouse IgM (Invitrogen, A10680, 2 μg/ml) or AF555 goat anti-mouse IgM (Thermo Fisher, A28180, 2 μg/ml; 2 hours, 4 °C). After cells' exposure to anti-thrombin III (AT III; Abcam, AB62542, 10 μg/ml; 2 hours, RT), 19 19. AT III was stained with anti-AT III (Invitrogen, MA534940, 5 μg/ml; overnight, 4 °C) and revealed by AF647 goat anti-mouse IgG (Invitrogen, A-21235, 2 μg/ml; 1 hour, RT). Nuclei were stained with 4′,6-diamidino-2-phenylindole (Sigma, D9542, 2 μg/ml). The whole slides were scanned with a Zeiss Axioscan Z1 (20×/numerical aperture, 0.8), and percentage area of staining, normalized to nuclei number and expressed in μm 2 of positive areas, was quantified using ImageJ software (NIH, version 1.52, 2018). Illustrative photomicrographs were performed with confocal microscopes (Spinning Disk, Zeiss Axio Observer Z1-Yokogawa CSU-X1, and ZEISS CLSM 710).
Laboux T., Maanaoui M., Allain F., Boulanger E., Denys A., Gibier J.B., Glowacki F., Grolaux G., Grunenwald A., Howsam M., Lancel S., Lebas C., Lopez B., Roumenina L., Provôt F., Gnemmi V, & Frimat M. (2023). Hemolysis is associated with altered heparan sulfate of the endothelial glycocalyx and with local complement activation in thrombotic microangiopathies. Kidney international, 104(2).
+ Open protocol
+ Expand
7

Immunofluorescence Staining of Cardiomyocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cardiomyocytes were fixed with pre-chilled methanol for 10 minutes, then washed twice using PBST (0.1% tween-20 in 1×PBS) with 5 minutes intervals. Cells were blocked for 1 hour at room temperature with blocking buffer (5% BSA in PBST) and incubated with primary antibodies overnight at 4°C. The primary antibodies were: Detyrosinated α-tubulin (Abcam, ab48389, used at 1:200), α-tubulin (CST, 3873S, used at 1:200), MARK4 (Abcam, ab124267, used at 1:200), APC anti-mouse CD45 (BioLegend, 103112, used at 1:200) and rabbit IgG isotype control (Novus Biologicals, NB810-56910, used at 1:2000). The cells were then washed with PBST and incubated with secondary antibody for 1 hour at room temperature. The secondary antibodies were: AF488 donkey anti-rabbit IgG (Invitrogen, A21206, used at 1:200), AF647 goat anti-mouse IgG (Invitrogen, A21236, used at 1:200), AF647 goat anti-rat IgG (Invitrogen, A21247, used at 1:200). DAPI (Sigma, 10236276001, used at 1:1000) was used. Confocal images were obtained by Leica SP5 Confocal Laser Scanning Microscope, collected by LAS AF software (2.7.3.9723), and analyzed by ImageJ (v2.0) analyze tools.
Yu X., Chen X., Amrute-Nayak M., Allgeyer E., Zhao A., Chenoweth H., Clement M., Harrison J., Doreth C., Sirinakis G., Krieg T., Zhou H., Huang H., Tokuraku K., St Johnston D., Mallat Z, & Li X. (2021). MARK4 controls ischaemic heart failure through microtubule detyrosination. Nature, 594(7864), 560-565.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!