Glyburide d11

Metformin Hydrochloride (C₄H₁₁N₅·HCl, purity 100.0%), glyburide (C₂₃H₂₈ClN₃O₅S, purity > 99.9%), tolbutamide (C₁₂H₁₈N₂O₃S, purity 98%), enalapril maleate (C₂₀H₂₈N₂O₅·C₄H₄O₄, purity 99.8%), indapamide (C₁₆H₁₆ClN₃O₃S, purity 98.2%), nifedipine (C₁₇H₁₈N₂O₆, purity 99.8%), atorvastatin calcium (C₆₆H₆₈CaF₂N₄O₁₀·3H₂O, purity 94.9%), and reserpine (C₃₃H₄₀N₂O₉, purity > 99.9%) were purchased from national institutes for food and drug control (Beijing, China). Glyburide-d11 (C₂₃H₁₇ClD₁₁N₃O₅S, purity 98%) was purchased from Toronto Research Chemicals (Toronto, ON, Canada). Methanol (MeOH), acetonitrile (ACN), and methyl tert butyl ether (MTBE) were of HPLC grade and purchased from Fisher Scientific (Pittsburgh, PA, USA). Acetic acid was obtained from Guangzhou chemical reagent factory (Guangzhou, China). Ammonium acetate was purchased from Macklin (Shanghai, China). Ultrapure water was freshly prepared using the Milli-Q Advantage A10 system (Milli-Q Reference, Millipore, Boston, MA, USA). Blank rat plasma samples were harvested from healthy adult rats supplied by the Laboratory Animal Center of Sun Yat-sen University (Guangdong, China).