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2 protocols using methyl gallate

1

Compound Procurement for Phytochemical Analysis

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S-adenosyl-L-methionine (SAM) was purchased from Shenggong (Shanghai, China). Gallic acid, EC, (−)-epigallocatechin, ECG, and EGCG were purchased from Sigma-Aldrich (St. Louis, MO, USA). ECG3″Me, ECG4″Me, EGCG3″Me, and EGCG4″Me, were purchased from Nagara Science Co., Ltd. (Gifu, Japan). Myricetin and tamarixetin were obtained from BioBioPha Co., Ltd (Kunming, China), and caffeic acid, ferulic acid, isoferulic acid, 3-O-MethylGallic acid, 4-O-MethylGallic acid, methyl gallate, methyl 4-O-methylgallate, cyanidin 3-O galactoside chloride, peonidin 3-O-galactoside chloride, isoquercitrin, isorhamnetin 3-O-glucoside and syringetin were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Mearnsetin and laricitrin were from ChemFaces (Wuhan, China). Methyl 3-O-methylgallate was from MedChemExpress (Monmouth Junction, NJ, USA). Myricetin 3′, 4′-dimethyl ether was provided by Prof. Ai-Xia Chen of Shandong University, China.
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2

Evaluation of α-Glucosidase Inhibitors

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The α-Glucosidase (from Saccharomyces cerevisiae) was purchased from Sigma-Aldrich (St. Louis, MO, USA). The p-nitrophenyl-α-D-glucopyranoside (PNPG , ≥99%), acarbose (≥95%), ellagic acid (≥98%), gallic acid (≥98%), protocatehuic acid (≥98%), methyl gallate (≥98%), gallic acid ethyl ester (≥98%), kaempferol-3-Orobinobioside (≥98%) and astragalin (≥98%) reference substances were obtained from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China) . Chromatography grade formic acid (≥98%) and caffeic acid (≥98%) reference substance were purchased from Shanghai Aladdin Reagent Co., Ltd. (Shanghai, China) . Corilagin (≥98%), geraniin (≥98%) and rutin (≥98%) reference substances were obtained from Chengdu Push Biotechnology Co., Ltd. (Chengdu, China) . Isoquercitrin (≥98%) reference substance was purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China) .
Chromatography grade methanol and acetonitrile were obtained from Merck (Darmstadt, Germany). The ultrapure water was purified by Milli-Q 700 ultrapure water system (Merck Millipore, Germany).
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