BPA, BPB, BPS, BPF, BPAF, AO44S25, 4-OP, 4-NP, 4OH-TAM, sodium nitrite-15N, and SYPRO Orange dye were purchased from Sigma-Aldrich (Oakville, ON, Canada). HisPur Ni-NTA (nitrilotriacetic acid) magnetic beads and Zeba 7K spin desalting plates were purchased from Thermo Fisher Scientific (Whitby, ON, Canada). Human ERRγ open reading frame (ORF) clone (BC064700.1) and pET28-MHL vector were provided by Structural Genomics Consortium. BL21(DE3) competent E. coli was obtained from BioLabs Inc. (Whitby, ON, Canada). Antibody to histidine was purchased from Bioshop (Burlington, ON, Canada; TAG001.100) and was diluted with 5% skim milk at a dilution of 1:1000 for Western blotting. Horseradish peroxidase–linked anti-mouse immunoglobulin G secondary antibody (7076S) was purchased from Cell Signaling Technology (Beverly, Massachusetts, USA), which was used at a dilution of 1:20,000 in 5% skim milk.
Zeba 7k spin desalting plates
Manufactured by Thermo Fisher Scientific
Sourced in Canada
Zeba 7K spin desalting plates are a simple and effective tool for desalting and buffer exchange of protein samples. They use high-performance resin to rapidly remove salts, buffers, and other small molecules from protein samples via size exclusion chromatography.
Automatically generated - may contain errors
Lab products found in correlation
4oh tam, by Merck Group (2 mentions)
Ao44s25, by Merck Group (2 mentions)
Sodium nitrite 15n, by Merck Group (2 mentions)
Hispur ni nta nitrilotriacetic acid magnetic beads, by Thermo Fisher Scientific (2 mentions)
Horseradish peroxidase linked anti mouse immunoglobulin g secondary antibody 7076s, by Cell Signaling Technology (2 mentions)
Sypro orange dye, by Merck Group (2 mentions)
2 protocols using zeba 7k spin desalting plates
1
Recombinant Human ERRγ Purification
2
Recombinant Human ERRγ Purification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
BPA, BPB, BPS, BPF, BPAF, AO44S25, 4-OP, 4-NP, 4OH-TAM, sodium nitrite-15N, and SYPRO Orange dye were purchased from Sigma-Aldrich (Oakville, ON, Canada). HisPur Ni-NTA (nitrilotriacetic acid) magnetic beads and Zeba 7K spin desalting plates were purchased from Thermo Fisher Scientific (Whitby, ON, Canada). Human ERRγ open reading frame (ORF) clone (BC064700.1) and pET28-MHL vector were provided by Structural Genomics Consortium. BL21(DE3) competent E. coli was obtained from BioLabs Inc. (Whitby, ON, Canada). Antibody to histidine was purchased from Bioshop (Burlington, ON, Canada; TAG001.100) and was diluted with 5% skim milk at a dilution of 1:1000 for Western blotting. Horseradish peroxidase–linked anti-mouse immunoglobulin G secondary antibody (7076S) was purchased from Cell Signaling Technology (Beverly, Massachusetts, USA), which was used at a dilution of 1:20,000 in 5% skim milk.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!