LC–ESI-MS analysis was performed on a XEVO TQD triple quadruple instrument and mass spectrometer (Waters Corporation, Milford, MA01757 USA). 10 µL as injection volumes were applied to UPLC instrument with C-18 reverse phase column (ACQUITY UPLC - BEH C18 1.7 µm particlesize − 2.1 × 50 mm column). Handling, processing of the sample for LC-MS analysis and the parameters for analysis were accomplished using negative ion mode as described by Ibrahim et al. (2016 ). The Mass spectra detection range was between m/z 100–1000, using the Maslynx 4.1 software for data processing. Pure flavonoids obtained from Phytochemical and Plant Systematics Department, NRC, Giza, Egypt were used as authentics in this study. Correlation between their fragmentation pattern and mass spectra with the known peaks were performed.
Xevo tqd triple quadruple instrument and mass spectrometer
Manufactured by Waters Corporation
Sourced in United States
The XEVO TQD is a triple quadrupole instrument and mass spectrometer manufactured by Waters Corporation. It is designed to perform high-sensitivity and high-selectivity quantitative analysis of a wide range of analytes in complex matrices.
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Lab products found in correlation
2 protocols using xevo tqd triple quadruple instrument and mass spectrometer
1
LC-ESI-MS Analysis of Flavonoids
2
LC-ESI-MS Analysis of Flavonoids
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LC-ESI-MS analysis was performed on a XEVO TQD triple quadruple instrument and mass spectrometer (Waters Corporation, Milford, MA01757 USA). 10 µL as injection volumes were applied to UPLC instrument with C-18 reverse phase column (ACQUITY UPLC -BEH C18 1.7 µm particlesize -2.1 × 50 mm column). Handling, processing of the sample for LC-MS analysis and the parameters for analysis were accomplished using negative ion mode as described by Ibrahim et al. (2016) . The Mass spectra detection range was between m/z 100-1000, using the Maslynx 4.1 software for data processing. Pure flavonoids obtained from Phytochemical and Plant Systematics Department, NRC, Giza, Egypt were used as authentics in this study. Correlation between their fragmentation pattern and mass spectra with the known peaks were performed.
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