Ivis lumina 3 living image system

Ex vivo imaging was performed in the colorectal cancer cells (CT26) tumor-bearing BALB/C mice (tumor volume, around 500 mm³) receiving the intravenous injection of DiR solution, CPT-OA/CM and CPT-SS-OA/CM (labeled with DiR) at an equivalent CPT dose of 10 mg/kg (DiR, 0.1 mg/kg). At the indicated times (12, 24, and 48 h), the mice were sacrificed, and the tumor, spleen, heart, lungs, liver, and kidneys were excised and imaged using an IVIS Lumina III living image system (PerkinElmer) with the excitation at 730 nm and emission at 790 nm. The mean fluorescence intensity of each organ was determined and compared.

The biodistribution of Cy5-labeled NP_Tyr-C9AP was analyzed in a bilateral tumor model. Because B16-F10 cells can produce abundant melanin that interferes with the in vivo detection of Cy5 signal, the bilateral tumor model was established by subcutaneously inoculating 1.5 × 10⁶ YUMM1.7 melanoma cells into the right flank of C57BL/6 mice and 5 × 10⁶ Panc02 pancreatic tumor cells into the left flank. When the melanoma volume reached 400–500 mm³, the mice were intravenously injected Cy5-labeled NP_Tyr-C9AP at a dose of 1 mg Cy5-labeled pTyr-C9AP per kg body weight. Twelve hours later, the YUMM1.7 melanoma, Panc02 pancreatic tumor and normal organs were collected, and were analyzed using IVIS Lumina III Living Image system (PerkinElmer, MA, USA). The data were collected using Living image software v4.4 (PerkinElmer).